Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605125526_agh8 ATGTGGGGCAGGTCAAGACCTAGGAAGAATGG(+CAGTGT)GATCCAGGCCAGCCTTGAGCAAAGTACCTGG NGG 10 8 17 8 24 0 100 5 10
    240605125525_6d7h AAGGTCTAAATCTGCTCCACCCTCTGAGCGAG(+CAGTGT)GTACCCTCACTTCAACCTGCCACACATTGCT NGG 10 8 17 8 24 0 100 5 10
    240605125523_ti8f GTGCCAGCAATGTGTGGCAGGTTGAAGTGAGG(+CAGTGT)GTACCTCGCTCAGAGGGTGGAGCAGATTTAG NGG 10 8 17 8 24 0 100 5 10
    240605125522_65sx CCATGACTGCCAAGTCGCCTATTTTCTGTAAG(+CAGTGT)GTACCGGCTCTCCACACAGAAACCTCCACTC NGG 10 8 17 8 24 0 100 5 10
    240605125520_081c CTTCGGAGTGGAGGTTTCTGTGTGGAGAGCCG(+CAGTGT)GTACCTTACAGAAAATAGGCGACTTGGCAGT NGG 10 8 17 8 24 0 100 5 10
    240605125519_6ze5 CCAGCTTGGCATTAATTACCAAGCCCGCTTTG(+CAGTGT)GTACCCTGCTGGAGACCTGGCCTATGTACAG NGG 10 8 17 8 24 0 100 5 10
    240605125517_6eve GCTTGCTGTACATAGGCCAGGTCTCCAGCAGG(+CAGTGT)GTACCAAAGCGGGCTTGGTAATTAATGCCAA NGG 10 8 17 8 24 0 100 5 10
    240605125516_r570 TTATGGCTGCGGCGTGCTGGCGTAGAACATTG(+CAGTGT)GATCCGCCCCTAAGGTACTTGCGTGCCCGAC NGG 10 8 17 8 24 0 100 5 10
    240605125514_lss0 GGGGCGTCGGGCACGCAAGTACCTTAGGGGCG(+CAGTGT)GATCCAATGTTCTACGCCAGCACGCCGCAGC NGG 10 8 17 8 24 0 100 5 10
    240605125513_qndi CCCATCCTGTCAGGTTGGTGCGGAAGGGCTAG(+CAGTGT)GATCCTTGCCGCCCTTCCCTTCGGTGCCGGA NGG 10 8 17 8 24 0 100 5 10