Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605125542_p0b6 ATTTGGCACATTAACACCGCCCCGCTCCCTGG(+CAGTGT)GTACCGATCACACAGCACACAACCTTATCAC NGG 10 8 17 8 24 0 100 5 10
    240605125541_7meg TTGCTGATGCTCAGTGGTCCAGGCTACGCTGG(+CAGTGT)GATCCGTGATGGGGATATCCCAGAAGACACC NGG 10 8 17 8 24 0 100 5 10
    240605125539_pzb3 GGAATGGTGTCTTCTGGGATATCCCCATCACG(+CAGTGT)GATCCCAGCGTAGCCTGGACCACTGAGCATC NGG 10 8 17 8 24 0 100 5 10
    240605125538_joaq ACAGCAATCCATGGCTTTCCAGAAGAGGTGTG(+CAGTGT)GATCCAAAAGTGCCACCTCTATCTTAGTCAC NGG 10 8 17 8 24 0 100 5 10
    240605125536_oj2n TGCTGGTGACTAAGATAGAGGTGGCACTTTTG(+CAGTGT)GATCCACACCTCTTCTGGAAAGCCATGGATT NGG 10 8 17 8 24 0 100 5 10
    240605125535_czg4 TCAAGTGCCAAAGCTGTCTCGTGAGCTTCCAG(+CAGTGT)GATCCTGACAGGCCTGGAGGGAACTGCAAAG NGG 10 8 17 8 24 0 100 5 10
    240605125533_rdlw TGGCCCTTTGCAGTTCCCTCCAGGCCTGTCAG(+CAGTGT)GATCCTGGAAGCTCACGAGACAGCTTTGGCA NGG 10 8 17 8 24 0 100 5 10
    240605125532_f9he CAGGTGTTCTTTCAGGCTGGTCAGGAGCTGTG(+CAGTGT)GTACCAGGGACAAGGCCTGCCTCTTGTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605125530_px0d AAAACAAAGACAAGAGGCAGGCCTTGTCCCTG(+CAGTGT)GTACCACAGCTCCTGACCAGCCTGAAAGAAC NGG 10 8 17 8 24 0 100 5 10
    240605125528_2b0d GGACACCAGGTACTTTGCTCAAGGCTGGCCTG(+CAGTGT)GATCCCATTCTTCCTAGGTCTTGACCTGCCC NGG 10 8 17 8 24 0 100 5 10