Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605125454_09z4 GAGCAACCATGCTTTGAAAAGAGGCTCTGAGG(+CAGTGT)GATCCTTACTGCAGGTCTTAGCTTTAGCGAT NGG 10 8 17 8 24 0 100 5 10
    240605125453_kdcy AAAAAGCTACAAACATTTCCAACAGACAAATG(+CAGTGT)GATCCTAGCTGCCCCATGCATGGTTCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605125452_6pmn CATGCTCCCAGAACCATGCATGGGGCAGCTAG(+CAGTGT)GATCCATTTGTCTGTTGGAAATGTTTGTAGC NGG 10 8 17 8 24 0 100 5 10
    240605125450_w86c TAGGGGCTGGAGCTGAGCTGCCTTCTGTCCTG(+CAGTGT)GTACCTGGAGGGAACGAGGATGTTAAGTGAT NGG 10 8 17 8 24 0 100 5 10
    240605125448_e07s AGTCAATCACTTAACATCCTCGTTCCCTCCAG(+CAGTGT)GTACCAGGACAGAAGGCAGCTCAGCTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605125447_iacb CTGCCCACTGTGCCTCAGCTGTGTGGAGCCTG(+CAGTGT)GTACCAGAGTCAGGCCCCAGAGAAGGGAGGA NGG 10 8 17 8 24 0 100 5 10
    240605125446_kgtj TTTTGTCCTCCCTTCTCTGGGGCCTGACTCTG(+CAGTGT)GTACCAGGCTCCACACAGCTGAGGCACAGTG NGG 10 8 17 8 24 0 100 5 10
    240605125444_8j2o GGGCATCTCCACCATTCTTACTGACGAACCTG(+CAGTGT)GTACCACTGGGAACACTACAGGCAGCTCCTA NGG 10 8 17 8 24 0 100 5 10
    240605125442_9uuq GAAGTTAGGAGCTGCCTGTAGTGTTCCCAGTG(+CAGTGT)GTACCAGGTTCGTCAGTAAGAATGGTGGAGA NGG 10 8 17 8 24 0 100 5 10
    240605125440_cvxy TTGTAAGAAGCATTTTAAGCAGCTGCTGTAGG(+CAGTGT)GTACCTTAAAAAAAAAACTTCCAGTAGAGTA NGG 10 8 17 8 24 0 100 5 10