Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605125253_kkq6 AAGGGGAGTTGGGTGGGTGGAGAAAGTAGCTG(+CAGTGT)GTACCAAAGGCAGGGGCGTGAATGGGCTGAA NGG 10 8 17 8 24 0 100 5 10
    240605125251_20nl AGATTGGGGCAGTGGGACAAGCTATAATCCTG(+CAGTGT)GTACCCAGGAGGCAGTGGTATGGAGATTTTG NGG 10 8 17 8 24 0 100 5 10
    240605125249_p8q6 GACCACAAAATCTCCATACCACTGCCTCCTGG(+CAGTGT)GTACCAGGATTATAGCTTGTCCCACTGCCCC NGG 10 8 17 8 24 0 100 5 10
    240605125248_r180 TGCAAAACAAAACAAAACAAAACAAAACAAAG(+CAGTGT)GATCCTGTATTGGCAGAGATGGTGGTAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605125247_5cjs ATGCATCTTTACCACCATCTCTGCCAATACAG(+CAGTGT)GATCCTTTGTTTTGTTTTGTTTTGTTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605125245_ei3k CCAGTTGTAATTGCTTTGAACCAAGCACCTCG(+CAGTGT)GATCCTGACTACTTTAAACACTGAGCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240605125244_w2w7 TACCACTGGAGCTCAGTGTTTAAAGTAGTCAG(+CAGTGT)GATCCGAGGTGCTTGGTTCAAAGCAATTACA NGG 10 8 17 8 24 0 100 5 10
    240605125243_5b5p GCATGTATGTCTGTGTTTCAGTAGCATGCTTG(+CAGTGT)GTACCCAAGGAAGCCAGAAAAAGGCATTAGA NGG 10 8 17 8 24 0 100 5 10
    240605125240_7723 GGGCATCTAATGCCTTTTTCTGGCTTCCTTGG(+CAGTGT)GTACCAAGCATGCTACTGAAACACAGACATA NGG 10 8 17 8 24 0 100 5 10
    240605125237_nxkd GCTCACGGCCATTGCAGCTCTTCCTTCCAGGG(+CAGTGT)GATCCAACTCCCTCTTGGGCCTCCACTGACA NGG 10 8 17 8 24 0 100 5 10