Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605125204_hatr AGAGATACCTGCCATCCCAAAAGCACCTAGAG(+CAGTGT)GTACCCACTGTGTGACAGCTCAGTAAAGGCC NGG 10 8 17 8 24 0 100 5 10
    240605125203_ue88 CAGGGGATTTCATCAAGAATCCCAACTATTTG(+CAGTGT)GTACCAGAGCCAAGCAAATGCAAGCAGAACA NGG 10 8 17 8 24 0 100 5 10
    240605125201_sbsx CCAAGTGTTCTGCTTGCATTTGCTTGGCTCTG(+CAGTGT)GTACCAAATAGTTGGGATTCTTGATGAAATC NGG 10 8 17 8 24 0 100 5 10
    240605125200_5wzy GAGCTGCTGCCACACTCGGGCCCAGCGAGGAG(+CAGTGT)GATCCGGGAAAGGCACCTCGGAACTCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240605125158_en7z CTTTGTGGCTGAGTTCCGAGGTGCCTTTCCCG(+CAGTGT)GATCCTCCTCGCTGGGCCCGAGTGTGGCAGC NGG 10 8 17 8 24 0 100 5 10
    240605125155_537b GTCTTCTCCCAATCACAGTTCCAGGGGCCAGG(+CAGTGT)GTACCTCCAGAGGACTTCTCAAGCTGGTTGG NGG 10 8 17 8 24 0 100 5 10
    240605125154_fo98 GAAACCCAACCAGCTTGAGAAGTCCTCTGGAG(+CAGTGT)GTACCCTGGCCCCTGGAACTGTGATTGGGAG NGG 10 8 17 8 24 0 100 5 10
    240605125152_yixw TATACTTGGGGACAGTTACAAGGGAGTCACAG(+CAGTGT)GTACCCTGTCCATGTCTGCGGCTGTGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240605125151_3224 GCAGGCTGCCCACAGCCGCAGACATGGACAGG(+CAGTGT)GTACCTGTGACTCCCTTGTAACTGTCCCCAA NGG 10 8 17 8 24 0 100 5 10
    240605125149_dwcn CAAGTCAGAATTCCTGGTAGGGGCGGTTTCAG(+CAGTGT)GATCCCACAGAAATGAAGGGACACAGTCTTC NGG 10 8 17 8 24 0 100 5 10