Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605125130_likv CCCAGAATTCTCATTCGCTGCGGAACGCCCTG(+CAGTGT)GTACCTGGCAGGTTCCCCGCCTGTCTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605125128_i4nv GAGAGAAACAGAGGGATAAAGAGGAGGCTCGG(+CAGTGT)GTACCACTATGGTTCCAGAGTCCTCTGCCCT NGG 10 8 17 8 24 0 100 5 10
    240605125127_a7dw GGTAAAGGGCAGAGGACTCTGGAACCATAGTG(+CAGTGT)GTACCCGAGCCTCCTCTTTATCCCTCTGTTT NGG 10 8 17 8 24 0 100 5 10
    240605125125_8bhf GGTCACAATTGTCCCTAAATCTAGTTCCAAGG(+CAGTGT)GATCCAGCATCTTCTGGCCTCCGTGGGCACC NGG 10 8 17 8 24 0 100 5 10
    240605125124_9g69 TGCCTGGTGCCCACGGAGGCCAGAAGATGCTG(+CAGTGT)GATCCCTTGGAACTAGATTTAGGGACAATTG NGG 10 8 17 8 24 0 100 5 10
    240605125122_eun4 CTTGGTTAAAATTAGTTCTCACTCCATGTATG(+CAGTGT)GATCCTTCAACTTCTCTTCAGAGAGAGAAGT NGG 10 8 17 8 24 0 100 5 10
    240605125121_3rm1 TGAGGACTTCTCTCTCTGAAGAGAAGTTGAAG(+CAGTGT)GATCCATACATGGAGTGAGAACTAATTTTAA NGG 10 8 17 8 24 0 100 5 10
    240605125120_4w7k TAAGTGAGACAAGAGCCCAGCCCAACCTCAGG(+CAGTGT)GATCCAACAGTCCACGGCCATCTCCTTAGCT NGG 10 8 17 8 24 0 100 5 10
    240605125119_gbp8 GGGACAGCTAAGGAGATGGCCGTGGACTGTTG(+CAGTGT)GATCCCTGAGGTTGGGCTGGGCTCTTGTCTC NGG 10 8 17 8 24 0 100 5 10
    240605125118_iolg TGTCCCTCTGACTGGATACAACCTAGTAAATG(+CAGTGT)GTACCTTCCAGCTGGGCCTGTTTCTGCTGCT NGG 10 8 17 8 24 0 100 5 10