Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605125045_no18 GGGATTTATGGGGTCCATAGCTTCTGTCTAGG(+CAGTGT)GATCCGGGCTAGTGTCCTTGGTGAGTTCCAG NGG 10 8 17 8 24 0 100 5 10
    240605125042_apgw TCTGTTCAATAGAGGCCACTTCCTTCCCAAGG(+CAGTGT)GATCCAAAACCCAAAACCTGCGGCTTCCACT NGG 10 8 17 8 24 0 100 5 10
    240605125041_l969 GTTTCAGTGGAAGCCGCAGGTTTTGGGTTTTG(+CAGTGT)GATCCCTTGGGAAGGAAGTGGCCTCTATTGA NGG 10 8 17 8 24 0 100 5 10
    240605125039_hci0 AAGTCCATTTCCCAGCCATACGCAGCTGTGGG(+CAGTGT)GATCCCAGGAGACACTTCAAGTGGAATGAAT NGG 10 8 17 8 24 0 100 5 10
    240605125037_x1p5 TTGAGATTCATTCCACTTGAAGTGTCTCCTGG(+CAGTGT)GATCCCCACAGCTGCGTATGGCTGGGAAATG NGG 10 8 17 8 24 0 100 5 10
    240605125035_jdib TGGAATGGCTGTCCTGTAGGCAGGTTGGGGTG(+CAGTGT)GTACCCTGGGGCTTCTGGGAATGGGGCTATG NGG 10 8 17 8 24 0 100 5 10
    240605125032_563i AACACCATAGCCCCATTCCCAGAAGCCCCAGG(+CAGTGT)GTACCACCCCAACCTGCCTACAGGACAGCCA NGG 10 8 17 8 24 0 100 5 10
    240605125030_bkxi GGGGGGCTCTCATCCCCTCCTCCACCCCAGAG(+CAGTGT)GATCCAAGTGAGCACCAGCTGACCTGCTCAG NGG 10 8 17 8 24 0 100 5 10
    240605125029_smgy CGGGACTGAGCAGGTCAGCTGGTGCTCACTTG(+CAGTGT)GATCCTCTGGGGTGGAGGAGGGGATGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240605125026_m1kq TGCTTCAAGCTGTGAGGAAGCCGCAGCCCAGG(+CAGTGT)GATCCCAAGCTTTTCGGGAGTGGCCTTGTTC NGG 10 8 17 8 24 0 100 5 10