Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605125116_zv74 GAAGGAGCAGCAGAAACAGGCCCAGCTGGAAG(+CAGTGT)GTACCATTTACTAGGTTGTATCCAGTCAGAG NGG 10 8 17 8 24 0 100 5 10
    240605125115_arp4 TTGTCCCCAGGATACAGCAGATGCTCAACAGG(+CAGTGT)GTACCAGAGACACTGTCTGTCTGGAGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240605125114_j386 CCGCCAGAGCCTCCAGACAGACAGTGTCTCTG(+CAGTGT)GTACCCTGTTGAGCATCTGCTGTATCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605125112_xkjs TTTTCCTTTCCAGAAACATGTTTGGGGCCGTG(+CAGTGT)GATCCGAACAGGCAACAGGGGTGCTCTGCCA NGG 10 8 17 8 24 0 100 5 10
    240605125111_hxec ATCTTTGGCAGAGCACCCCTGTTGCCTGTTCG(+CAGTGT)GATCCACGGCCCCAAACATGTTTCTGGAAAG NGG 10 8 17 8 24 0 100 5 10
    240605125109_8dlo CAAGGAAGGGCCCTCTGTCCCCTGATATGCTG(+CAGTGT)GTACCATTGTTTGTGTCCAGGGGATGCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605125108_4ndq GGTGAGCTGGCATCCCCTGGACACAAACAATG(+CAGTGT)GTACCAGCATATCAGGGGACAGAGGGCCCTT NGG 10 8 17 8 24 0 100 5 10
    240605125107_8thp CAGGCCAGGACCCACACAACTGATGGGGGCAG(+CAGTGT)GATCCCCAGTGATGGGTCCAGAGTGGGAATC NGG 10 8 17 8 24 0 100 5 10
    240605125104_4v22 GACTTGATTCCCACTCTGGACCCATCACTGGG(+CAGTGT)GATCCTGCCCCCATCAGTTGTGTGGGTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605125103_whb9 GGTCTCCCGAGAGCATGCCGAGTGTCCTGAGG(+CAGTGT)GATCCTCGGCTAGCCCCCATCTTCTACTTGA NGG 10 8 17 8 24 0 100 5 10