Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605125148_jhtg TGGGAGAAGACTGTGTCCCTTCATTTCTGTGG(+CAGTGT)GATCCTGAAACCGCCCCTACCAGGAATTCTG NGG 10 8 17 8 24 0 100 5 10
    240605125146_spnu CATCCAGGCCCCCAATCTTTTATATTGAAGGG(+CAGTGT)GATCCAACTTCCAGGATGAAAGGCAGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240605125145_75zu ACCAGGCTCTCTGCCTTTCATCCTGGAAGTTG(+CAGTGT)GATCCCCTTCAATATAAAAGATTGGGGGCCT NGG 10 8 17 8 24 0 100 5 10
    240605125143_9xxk GCTGAGCAAGAAAGGCTGTCTGAGCCATGGTG(+CAGTGT)GATCCCCAGGGAGGGAATGTCCCCTCCCCCC NGG 10 8 17 8 24 0 100 5 10
    240605125140_zweb GGGCAGGGGGGAGGGGACATTCCCTCCCTGGG(+CAGTGT)GATCCACCATGGCTCAGACAGCCTTTCTTGC NGG 10 8 17 8 24 0 100 5 10
    240605125139_0jtp GCCTACTTCTGGATCACCCTCATCCAGTGCTG(+CAGTGT)GTACCCCACAGCACTAAATCCAGGAACCCTG NGG 10 8 17 8 24 0 100 5 10
    240605125137_68gd AAGCCCAGGGTTCCTGGATTTAGTGCTGTGGG(+CAGTGT)GTACCAGCACTGGATGAGGGTGATCCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240605125136_p48j GGAAGCAGAAGGCAGAGCTGGCCCTGGTACTG(+CAGTGT)GTACCCCACCCTTCAGCGTAGCCCTTGGTGA NGG 10 8 17 8 24 0 100 5 10
    240605125134_ccb2 AGCTGTCACCAAGGGCTACGCTGAAGGGTGGG(+CAGTGT)GTACCAGTACCAGGGCCAGCTCTGCCTTCTG NGG 10 8 17 8 24 0 100 5 10
    240605125132_py1s AGAGAAAAGCAGACAGGCGGGGAACCTGCCAG(+CAGTGT)GTACCAGGGCGTTCCGCAGCGAATGAGAATT NGG 10 8 17 8 24 0 100 5 10