Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605125101_oe94 AGCCTTCAAGTAGAAGATGGGGGCTAGCCGAG(+CAGTGT)GATCCCTCAGGACACTCGGCATGCTCTCGGG NGG 10 8 17 8 24 0 100 5 10
    240605125059_ft0y GGAGGGAACCTTGGCAGCACTGGGCCTGTAGG(+CAGTGT)GATCCAGAGTTCTCAGGCAATAGAAGTGATC NGG 10 8 17 8 24 0 100 5 10
    240605125058_9di0 ACCCAGATCACTTCTATTGCCTGAGAACTCTG(+CAGTGT)GATCCCTACAGGCCCAGTGCTGCCAAGGTTC NGG 10 8 17 8 24 0 100 5 10
    240605125056_d0de GAGGGTGGTCTGTCGCATCACCACATTAGTGG(+CAGTGT)GTACCTGACAGTTACCTCATTGGCAGCCAAG NGG 10 8 17 8 24 0 100 5 10
    240605125055_c01o CTCACCTTGGCTGCCAATGAGGTAACTGTCAG(+CAGTGT)GTACCCACTAATGTGGTGATGCGACAGACCA NGG 10 8 17 8 24 0 100 5 10
    240605125053_qaot CTGCCCCTCTGACTCCTGCCCAGAAGTGGTGG(+CAGTGT)GTACCCCCCCACCCCGTGCCATTCATTGTCC NGG 10 8 17 8 24 0 100 5 10
    240605125051_xius GGGTGGGACAATGAATGGCACGGGGTGGGGGG(+CAGTGT)GTACCCACCACTTCTGGGCAGGAGTCAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605125050_1qeo GAGCTTCTTTGGTATCACCTGTCTATGTGCAG(+CAGTGT)GATCCTTCCAAAATGGAAGTAAACTACGCAG NGG 10 8 17 8 24 0 100 5 10
    240605125048_56zn GGGTGCTGCGTAGTTTACTTCCATTTTGGAAG(+CAGTGT)GATCCTGCACATAGACAGGTGATACCAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605125046_yx0q TGTGTCTGGAACTCACCAAGGACACTAGCCCG(+CAGTGT)GATCCCTAGACAGAAGCTATGGACCCCATAA NGG 10 8 17 8 24 0 100 5 10