Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605120334_0xv2 ACAGAGGAAGTGTTCTAGAGATAATCAAGGTG(+CAGTGT)GTACCTTGTGCTGTGGCTGGACTTGGTAGTG NGG 10 8 17 8 24 0 100 5 10
    240605120332_p0s1 TAAAAATTAAACTCCAACTGTCCAATGTCTGG(+CAGTGT)GATCCAACTCACAATCTTTTGGACAACTCCA NGG 10 8 17 8 24 0 100 5 10
    240605120331_6vww TCTTTTGGAGTTGTCCAAAAGATTGTGAGTTG(+CAGTGT)GATCCCAGACATTGGACAGTTGGAGTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240605120330_rrhs CAGCAGTAAAACCCTAACTAAGACAGAAGTTG(+CAGTGT)GTACCAGGAGTGGGGTAATGCTGTGATAGGC NGG 10 8 17 8 24 0 100 5 10
    240605120328_cy3i GTCAGGCCTATCACAGCATTACCCCACTCCTG(+CAGTGT)GTACCAACTTCTGTCTTAGTTAGGGTTTTAC NGG 10 8 17 8 24 0 100 5 10
    240605120327_z6ld GCCCTTGGAGGAGCCCAGAAGATCATGAGTTG(+CAGTGT)GATCCCAGACGTTGGACAGTTGGAATTTAAT NGG 10 8 17 8 24 0 100 5 10
    240605120325_us1r GAAAAATTAAATTCCAACTGTCCAACGTCTGG(+CAGTGT)GATCCAACTCATGATCTTCTGGGCTCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240605120324_w0mr ACAGAGGAAGTGTTCTAGAGATAATCAAGGTG(+CAGTGT)GTACCTTGTGCTGTGGCTGGACTTGGTAGTG NGG 10 8 17 8 24 0 100 5 10
    240605120322_rabi TTACACACTACCAAGTCCAGCCACAGCACAAG(+CAGTGT)GTACCACCTTGATTATCTCTAGAACACTTCC NGG 10 8 17 8 24 0 100 5 10
    240605120321_sr0i TTAATCCTCTAGGAGTTAATCCTCTAGGACTG(+CAGTGT)GTACCTACTCAGTTATGGCAAATAGACGTTA NGG 10 8 17 8 24 0 100 5 10