Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605120253_h51e AATAGTTTTATTTCACACCTTGTTTCTAGCTG(+CAGTGT)GTACCACTTGCATGATGCAACCAAAAAACAT NGG 10 8 17 8 24 0 100 5 10
    240605120251_c58b TCTTTTGGAGTTGTCCAAAAGATCGTGAGTTG(+CAGTGT)GATCCCAGACATTGGACAGTTGGAGTTTCAT NGG 10 8 17 8 24 0 100 5 10
    240605120250_mhp3 TAAAAATGAAACTCCAACTGTCCAATGTCTGG(+CAGTGT)GATCCAACTCACGATCTTTTGGACAACTCCA NGG 10 8 17 8 24 0 100 5 10
    240605120249_n0xv GTGCTGTGGCTGGACTTGGTAACGTGTAAGAG(+CAGTGT)GTACCCAGATGGTACTGGTTTTGAAAGCATA NGG 10 8 17 8 24 0 100 5 10
    240605120247_fv6o CCTTTTATGCTTTCAAAACCAGTACCATCTGG(+CAGTGT)GTACCTCTTACACGTTACCAAGTCCAGCCAC NGG 10 8 17 8 24 0 100 5 10
    240605120246_1b7f GCCCTTGGAGGAGCCCAAAGGATCGTGAGTTG(+CAGTGT)GATCCCATACTTTGGATAGTTGGAGTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240605120244_5a9j CCAAAATTAAACTCCAACTATCCAAAGTATGG(+CAGTGT)GATCCAACTCACGATCCTTTGGGCTCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240605120242_ablc TTTTTGGGCTTATTTTGAAGCCACTCCCCAAG(+CAGTGT)GATCCACATTTATTCATGAAAGCAGATATCC NGG 10 8 17 8 24 0 100 5 10
    240605120241_lrhn GGTCTGGATATCTGCTTTCATGAATAAATGTG(+CAGTGT)GATCCTTGGGGAGTGGCTTCAAAATAAGCCC NGG 10 8 17 8 24 0 100 5 10
    240605120239_sy4u AAATTCAATTTCATAGACTACTGCCCAGCTAG(+CAGTGT)GATCCAGGTCTCAAGCACACAGATGCCTCGC NGG 10 8 17 8 24 0 100 5 10