Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605120205_0hdh GACAAGGCACAGGCTCCGTAGCAGTGGCTGAG(+CAGTGT)GATCCAAATCCCGCACGGGCTTCAGACGACT NGG 10 8 17 8 24 0 100 5 10
    240605120204_lngs TTAATGTGTTTCTTTGCATAGATGTTACTTAG(+CAGTGT)GTACCGGAATGGGAGAGCAGCGGGGGTGATG NGG 10 8 17 8 24 0 100 5 10
    240605120202_1d12 TGGATCATCACCCCCGCTGCTCTCCCATTCCG(+CAGTGT)GTACCTAAGTAACATCTATGCAAAGAAACAC NGG 10 8 17 8 24 0 100 5 10
    240605120201_02i6 TGTGATACCAGCATGCCCTGATACGCCGTTAG(+CAGTGT)GTACCACAGGTCTACTACTGGGAGAGCTATG NGG 10 8 17 8 24 0 100 5 10
    240605120159_w2sc AGGGTCATAGCTCTCCCAGTAGTAGACCTGTG(+CAGTGT)GTACCTAACGGCGTATCAGGGCATGCTGGTA NGG 10 8 17 8 24 0 100 5 10
    240605120157_uiic ACTTTCTATTCCAACCTCCCTCTACACCAGCG(+CAGTGT)GTACCCAACCTTCCTCATGCTGCCACCCTTT NGG 10 8 17 8 24 0 100 5 10
    240605120155_anzp GTACTAAAGGGTGGCAGCATGAGGAAGGTTGG(+CAGTGT)GTACCGCTGGTGTAGAGGGAGGTTGGAATAG NGG 10 8 17 8 24 0 100 5 10
    240605120152_255e GAGAGAGAGGGGTGGACTGAACATGGCCGGGG(+CAGTGT)GATCCGTGAGACGGAGAGGAAACTGGCACAG NGG 10 8 17 8 24 0 100 5 10
    240605120151_cmmu CTATCCTGTGCCAGTTTCCTCTCCGTCTCACG(+CAGTGT)GATCCCCCGGCCATGTTCAGTCCACCCCTCT NGG 10 8 17 8 24 0 100 5 10
    240605120149_knq7 TTAAAGAGTAACATCTACCTTGCAGTGGCCAG(+CAGTGT)GATCCTAGGACCTCTCACTGACTAGAGCGCT NGG 10 8 17 8 24 0 100 5 10