Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605120238_knsq AGTTAGCGAGGCATCTGTGTGCTTGAGACCTG(+CAGTGT)GATCCTAGCTGGGCAGTAGTCTATGAAATTG NGG 10 8 17 8 24 0 100 5 10
    240605120236_zm7l GCCCAAGTTTCTCTATCAGCCACTCCCACCAG(+CAGTGT)GATCCAGCCCCTCAGAGGCACTCAGCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240605120234_5fru GGACAGAGGAGCTGAGTGCCTCTGAGGGGCTG(+CAGTGT)GATCCTGGTGGGAGTGGCTGATAGAGAAACT NGG 10 8 17 8 24 0 100 5 10
    240605120232_c6uw GAGAGGACTCTGAAGCATCCTAGCTGCCCTGG(+CAGTGT)GTACCCAGCAACACTGCTAAACGGCTCCTGG NGG 10 8 17 8 24 0 100 5 10
    240605120230_86l8 GTTTGCCAGGAGCCGTTTAGCAGTGTTGCTGG(+CAGTGT)GTACCCAGGGCAGCTAGGATGCTTCAGAGTC NGG 10 8 17 8 24 0 100 5 10
    240605120229_2n8x AGAGGCACTGCCTCTACACTCATAAATTTTTG(+CAGTGT)GTACCAAACTTCTTAAAAACCAGATGGTTAA NGG 10 8 17 8 24 0 100 5 10
    240605120227_xmi8 TTTTTTTAACCATCTGGTTTTTAAGAAGTTTG(+CAGTGT)GTACCAAAAATTTATGAGTGTAGAGGCAGTG NGG 10 8 17 8 24 0 100 5 10
    240605120226_xfok TTTAAAATATAGGTTACCCTGCTACCCTGCTG(+CAGTGT)GATCCTGAGCCTGGAGGCCTGCAGATCCATC NGG 10 8 17 8 24 0 100 5 10
    240605120225_ftxh GCCCGGATGGATCTGCAGGCCTCCAGGCTCAG(+CAGTGT)GATCCAGCAGGGTAGCAGGGTAACCTATATT NGG 10 8 17 8 24 0 100 5 10
    240605120223_040g GGCAACTGGGGGGAGGTGGGGTCAGGAACCCG(+CAGTGT)GATCCACCAAGAGGACAGTTCCCAAAGAGGA NGG 10 8 17 8 24 0 100 5 10