Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605120221_g0hg CTCCCTCCTCTTTGGGAACTGTCCTCTTGGTG(+CAGTGT)GATCCGGGTTCCTGACCCCACCTCCCCCCAG NGG 10 8 17 8 24 0 100 5 10
    240605120220_f46b GTGGAGGTGTCTTTAACAAACACTTTACTTTG(+CAGTGT)GTACCAGCACTCAGGTGACACGGCTCAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605120219_4z2s AAGGGCCTCTGAGCCGTGTCACCTGAGTGCTG(+CAGTGT)GTACCAAAGTAAAGTGTTTGTTAAAGACACC NGG 10 8 17 8 24 0 100 5 10
    240605120217_21eq TCACCATTCCTAACAGAAGCACTGCACTGTTG(+CAGTGT)GTACCGAGAAGGGTGGAAGACAGGAGGGGGC NGG 10 8 17 8 24 0 100 5 10
    240605120216_jv54 CCCCCGCCCCCTCCTGTCTTCCACCCTTCTCG(+CAGTGT)GTACCAACAGTGCAGTGCTTCTGTTAGGAAT NGG 10 8 17 8 24 0 100 5 10
    240605120213_hjki ACGGCTCTTTAGGACAACACCACAAACCCAGG(+CAGTGT)GATCCCCAGTGGGGAGGAGGGAACAGATCTT NGG 10 8 17 8 24 0 100 5 10
    240605120211_jw38 CATAAAAGATCTGTTCCCTCCTCCCCACTGGG(+CAGTGT)GATCCCTGGGTTTGTGGTGTTGTCCTAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605120209_ww3z ACCAGGACAATGTCACCTCAGGATGGGACTGG(+CAGTGT)GATCCCTGCAAGATGGTTCTACATGGAACCC NGG 10 8 17 8 24 0 100 5 10
    240605120208_uizm GAGCCGGGTTCCATGTAGAACCATCTTGCAGG(+CAGTGT)GATCCCAGTCCCATCCTGAGGTGACATTGTC NGG 10 8 17 8 24 0 100 5 10
    240605120207_eux3 TTTCCAGTCGTCTGAAGCCCGTGCGGGATTTG(+CAGTGT)GATCCTCAGCCACTGCTACGGAGCCTGTGCC NGG 10 8 17 8 24 0 100 5 10