Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605120307_4cre AACAGTTTTATTTCACACCTTGTTTCTAGCTG(+CAGTGT)GTACCACTTGCATGATGCAACCAAAAAACAT NGG 10 8 17 8 24 0 100 5 10
    240605120305_hjt4 TCTTTTGGAGTTGTCCAAAAGATTGTGAGTTG(+CAGTGT)GATCCCAGACATTGGACAGTTGGAGTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240605120304_736o TAAAAATTAAACTCCAACTGTCCAATGTCTGG(+CAGTGT)GATCCAACTCACAATCTTTTGGACAACTCCA NGG 10 8 17 8 24 0 100 5 10
    240605120302_a6ij TATCTTGTGAACTGTCACTGCCATGGAGCAAG(+CAGTGT)GATCCCACCTCAGTGTTGAGACATCAGAGCT NGG 10 8 17 8 24 0 100 5 10
    240605120300_ycx7 GACCCAGCTCTGATGTCTCAACACTGAGGTGG(+CAGTGT)GATCCTTGCTCCATGGCAGTGACAGTTCACA NGG 10 8 17 8 24 0 100 5 10
    240605120259_n9kg ACAGAGGAAGTGTTCTAGAGATAATCAAGGTG(+CAGTGT)GTACCTTGTGCTGTGGCTGGACTTGGTAGTG NGG 10 8 17 8 24 0 100 5 10
    240605120258_ikak TTACACACTACCAAGTCCAGCCACAGCACAAG(+CAGTGT)GTACCACCTTGATTATCTCTAGAACACTTCC NGG 10 8 17 8 24 0 100 5 10
    240605120256_sawh TTAATCCTCTAGGAGTTAATCCTCTAGGACTG(+CAGTGT)GTACCTACTCAGTTATGGCAAGTAGACGTTA NGG 10 8 17 8 24 0 100 5 10
    240605120255_qqt7 ATAAGTAACGTCTACTTGCCATAACTGAGTAG(+CAGTGT)GTACCAGTCCTAGAGGATTAACTCCTAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605120254_vmzt GCTCAATGTTTTTTGGTTGCATCATGCAAGTG(+CAGTGT)GTACCAGCTAGAAACAAGGTGTGAAATAAAA NGG 10 8 17 8 24 0 100 5 10