Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605111232_tt5s AAGAGCACCACCCAATCACAAGCCTGGGGTTG(+CAGTGT)GATCCATGCCAGCCAAGGACTATGATTTCCA NGG 10 8 17 8 24 0 100 5 10
    240605111230_75pn AGCAGTGGAAATCATAGTCCTTGGCTGGCATG(+CAGTGT)GATCCAACCCCAGGCTTGTGATTGGGTGGTG NGG 10 8 17 8 24 0 100 5 10
    240605111229_wyuw GCTTGGCTACCTGTAGACTCTAATCCTAAGTG(+CAGTGT)GATCCAGAATTGCTTTCCCAAGTCTAACTTC NGG 10 8 17 8 24 0 100 5 10
    240605111228_9ucd GAACTGAAGTTAGACTTGGGAAAGCAATTCTG(+CAGTGT)GATCCACTTAGGATTAGAGTCTACAGGTAGC NGG 10 8 17 8 24 0 100 5 10
    240605111226_wtus AACCTAGAAACTGTAGGTGGGCATATATCAGG(+CAGTGT)GTACCTATGCCACCGCCAACTTCTCCTTTGT NGG 10 8 17 8 24 0 100 5 10
    240605111225_puxn CTTGGACAAAGGAGAAGTTGGCGGTGGCATAG(+CAGTGT)GTACCCTGATATATGCCCACCTACAGTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605111223_u7fp AATGTACTAAATGATTCTCCTCCTCTCTCCTG(+CAGTGT)GTACCTGGAGACCCCTGGCTATCATGGGATT NGG 10 8 17 8 24 0 100 5 10
    240605111221_tnlx TCAGTAATCCCATGATAGCCAGGGGTCTCCAG(+CAGTGT)GTACCAGGAGAGAGGAGGAGAATCATTTAGT NGG 10 8 17 8 24 0 100 5 10
    240605111220_69l7 ACTCAGAAAAGAACCATAGGCACACACGTGTG(+CAGTGT)GATCCTATATCTTGATATTATATAGATACAC NGG 10 8 17 8 24 0 100 5 10
    240605111219_q96y GGATGGTGTATCTATATAATATCAAGATATAG(+CAGTGT)GATCCACACGTGTGTGCCTATGGTTCTTTTC NGG 10 8 17 8 24 0 100 5 10