Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605111248_jtob AATCCCAGGGAACCATTCTAGGTCTCTTCTTG(+CAGTGT)GATCCACAGGCCCGATGTCTATGCTCCGGTG NGG 10 8 17 8 24 0 100 5 10
    240605111247_sis1 GGAAACACCGGAGCATAGACATCGGGCCTGTG(+CAGTGT)GATCCAAGAAGAGACCTAGAATGGTTCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605111245_4541 AATCCCAGGGAACCGTTCTAGGTCTCTTCTTG(+CAGTGT)GATCCACAGGCCCGATGTCTATGCCCCGGTG NGG 10 8 17 8 24 0 100 5 10
    240605111243_iuq5 GGGAACACCGGGGCATAGACATCGGGCCTGTG(+CAGTGT)GATCCAAGAAGAGACCTAGAACGGTTCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605111242_51i5 AATCCCAGGGAACCGTTCTAGGTCTCTTCTTG(+CAGTGT)GATCCACAGGCCCGATGTCTATGCCCCGGTG NGG 10 8 17 8 24 0 100 5 10
    240605111240_dy61 GGGAACACCGGGGCATAGACATCGGGCCTGTG(+CAGTGT)GATCCAAGAAGAGACCTAGAACGGTTCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605111238_c0ga TAGATGGGCAGCAATGGGCAGTTGCAACCAAG(+CAGTGT)GTACCCAGCAGTGTCTTCTACATCACCTATA NGG 10 8 17 8 24 0 100 5 10
    240605111237_ar2i CTGGATATAGGTGATGTAGAAGACACTGCTGG(+CAGTGT)GTACCTTGGTTGCAACTGCCCATTGCTGCCC NGG 10 8 17 8 24 0 100 5 10
    240605111235_wv4j GACAGGATGGGCTCCACACGTACCTGTGTTGG(+CAGTGT)GATCCCCACATTCGCTGTAACCTATCTGTAG NGG 10 8 17 8 24 0 100 5 10
    240605111233_hmuj GTGTGCTACAGATAGGTTACAGCGAATGTGGG(+CAGTGT)GATCCCAACACAGGTACGTGTGGAGCCCATC NGG 10 8 17 8 24 0 100 5 10