Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605111317_i3kh CCATTTCAAGGTGCAGTGTGAAGTATGACTAG(+CAGTGT)GTACCTCTCCATTTATTGAGGCTGGAACAGG NGG 10 8 17 8 24 0 100 5 10
    240605111316_uj1p GATTTCCTGTTCCAGCCTCAATAAATGGAGAG(+CAGTGT)GTACCTAGTCATACTTCACACTGCACCTTGA NGG 10 8 17 8 24 0 100 5 10
    240605111315_1fpv CTTCAGCTGGAGTGTGCTCTGCTGGTGCAGTG(+CAGTGT)GATCCTAGTGTGGTTCCCAGTACTCAACCTG NGG 10 8 17 8 24 0 100 5 10
    240605111314_s1v3 CCAACCAGGTTGAGTACTGGGAACCACACTAG(+CAGTGT)GATCCACTGCACCAGCAGAGCACACTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605111312_qj3d AGAGACAAAGGACACAGGAACCCGGCTGACTG(+CAGTGT)GTACCAAAGTGCACTCTCTTCAGGTGCCTCT NGG 10 8 17 8 24 0 100 5 10
    240605111311_e1j9 TGTGTAGAGGCACCTGAAGAGAGTGCACTTTG(+CAGTGT)GTACCAGTCAGCCGGGTTCCTGTGTCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605111309_owea TGTCTTTATTCTAATGTCTTTGGTGTGGGAAG(+CAGTGT)GTACCTCATAGGCTGCCAAAAGAGAAATGCA NGG 10 8 17 8 24 0 100 5 10
    240605111308_job7 ATGTTTGCATTTCTCTTTTGGCAGCCTATGAG(+CAGTGT)GTACCTTCCCACACCAAAGACATTAGAATAA NGG 10 8 17 8 24 0 100 5 10
    240605111307_tqwc TGGGTATGGATGGAGCCACACTCCTATGCAAG(+CAGTGT)GATCCATTTCCATGCAAGATTGTCAATTCTT NGG 10 8 17 8 24 0 100 5 10
    240605111306_395h TCTGGAAGAATTGACAATCTTGCATGGAAATG(+CAGTGT)GATCCTTGCATAGGAGTGTGGCTCCATCCAT NGG 10 8 17 8 24 0 100 5 10