Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605111401_ah9e CACTGCAAGGTAGTATTTACCTGATCACAAAG(+CAGTGT)GATCCTTTGAGGCTCTACCACATATTCAAAT NGG 10 8 17 8 24 0 100 5 10
    240605111400_y507 CTAGAATTTGAATATGTGGTAGAGCCTCAAAG(+CAGTGT)GATCCTTTGTGATCAGGTAAATACTACCTTG NGG 10 8 17 8 24 0 100 5 10
    240605111359_ibnl GGCTTAGTTTTTATTTAATAAGATATTTAATG(+CAGTGT)GATCCATTTTTATCAAGGTCAGAAAACATTT NGG 10 8 17 8 24 0 100 5 10
    240605111357_rsyi ATTTAAAATGTTTTCTGACCTTGATAAAAATG(+CAGTGT)GATCCATTAAATATCTTATTAAATAAAAACT NGG 10 8 17 8 24 0 100 5 10
    240605111356_unus AGGACAAAGGCTGGAAACTTCAGATGAACTTG(+CAGTGT)GTACCCACTGAGACTTCCCTGCTGAAAGACA NGG 10 8 17 8 24 0 100 5 10
    240605111354_rngw ACCACTGTCTTTCAGCAGGGAAGTCTCAGTGG(+CAGTGT)GTACCAAGTTCATCTGAAGTTTCCAGCCTTT NGG 10 8 17 8 24 0 100 5 10
    240605111352_2hf6 AAGAAGCCCGGCGGAGCTTTAGGTGGCCACAG(+CAGTGT)GTACCTGCTGGCAGTGCTGTCACATTTAAGG NGG 10 8 17 8 24 0 100 5 10
    240605111351_ado5 CCTTGCCTTAAATGTGACAGCACTGCCAGCAG(+CAGTGT)GTACCTGTGGCCACCTAAAGCTCCGCCGGGC NGG 10 8 17 8 24 0 100 5 10
    240605111350_hfkz ACTTGGCTGAGGAGACTCGGCTTAGGAAGCAG(+CAGTGT)GTACCTGACTTTTGAGAAAACCCACTAGCTT NGG 10 8 17 8 24 0 100 5 10
    240605111348_jo7a AACAGAAGCTAGTGGGTTTTCTCAAAAGTCAG(+CAGTGT)GTACCTGCTTCCTAAGCCGAGTCTCCTCAGC NGG 10 8 17 8 24 0 100 5 10