Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605111417_85ty CAGCTGACAGTGGTGCACCTTTCACAGCCAGG(+CAGTGT)GATCCATGGGGCAGGGGACAGGTGCAGGCGC NGG 10 8 17 8 24 0 100 5 10
    240605111414_bujb CAGCAGCGCCTGCACCTGTCCCCTGCCCCATG(+CAGTGT)GATCCCTGGCTGTGAAAGGTGCACCACTGTC NGG 10 8 17 8 24 0 100 5 10
    240605111413_1umd TTTCAGGAACAATGAATGGTAGGACTCTATAG(+CAGTGT)GTACCCATGTTTCTCTTACTAAACTGTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605111411_iuru ACTACAACAACAGTTTAGTAAGAGAAACATGG(+CAGTGT)GTACCTATAGAGTCCTACCATTCATTGTTCC NGG 10 8 17 8 24 0 100 5 10
    240605111410_h0md TAAATCCTGTCTACAAAAATTATGTCATACAG(+CAGTGT)GTACCATTTGTTCTTTCTCTTTCCAAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240605111409_xs6x AATGGACTCTTTGGAAAGAGAAAGAACAAATG(+CAGTGT)GTACCTGTATGACATAATTTTTGTAGACAGG NGG 10 8 17 8 24 0 100 5 10
    240605111407_he7w GAGACGAGTTTCTGAAAATATACTGCACAATG(+CAGTGT)GATCCTAGGAGAGCCCAATCAAAGGCCTACT NGG 10 8 17 8 24 0 100 5 10
    240605111406_2mub AAGAGAGTAGGCCTTTGATTGGGCTCTCCTAG(+CAGTGT)GATCCATTGTGCAGTATATTTTCAGAAACTC NGG 10 8 17 8 24 0 100 5 10
    240605111404_g2yb TGGTGTACCAGGCACTATTATTCTTACAGAGG(+CAGTGT)GTACCATGAAAGTTAGATCCAGGTGCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605111403_du18 GGAGCCAAAGGCACCTGGATCTAACTTTCATG(+CAGTGT)GTACCCTCTGTAAGAATAATAGTGCCTGGTA NGG 10 8 17 8 24 0 100 5 10