Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605111508_fdfg AGAGCAGCCTCAGAGAGGCTTGTCCTAGTCAG(+CAGTGT)GATCCAGGATCTTGGCTGGGGCCTGCTTCTT NGG 10 8 17 8 24 0 100 5 10
    240605111506_2544 GAACAAAGAAGCAGGCCCCAGCCAAGATCCTG(+CAGTGT)GATCCTGACTAGGACAAGCCTCTCTGAGGCT NGG 10 8 17 8 24 0 100 5 10
    240605111505_q1qw CCAGGCATGCTCCTGCCTAGGTGAAATGAAAG(+CAGTGT)GATCCACATACTGGCACATGACATGATCCTA NGG 10 8 17 8 24 0 100 5 10
    240605111504_xib9 CTGAATAGGATCATGTCATGTGCCAGTATGTG(+CAGTGT)GATCCTTTCATTTCACCTAGGCAGGAGCATG NGG 10 8 17 8 24 0 100 5 10
    240605111501_9soi CCTTTTAGCTGCCATCTGCCAAGCACACCTGG(+CAGTGT)GATCCACCAGTTCTGGCAATGCTGTGACAGT NGG 10 8 17 8 24 0 100 5 10
    240605111500_h61s CAGTCACTGTCACAGCATTGCCAGAACTGGTG(+CAGTGT)GATCCCAGGTGTGCTTGGCAGATGGCAGCTA NGG 10 8 17 8 24 0 100 5 10
    240605111457_cj8s AAAAAAAAAAAATTCCCGGGAGCAACTGCCTG(+CAGTGT)GATCCTTTCCCTACAGTCGCTCGATCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240605111456_lqlr CTGTCTGGGAGATCGAGCGACTGTAGGGAAAG(+CAGTGT)GATCCAGGCAGTTGCTCCCGGGAATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605111455_qhcx CCTTGGTTCTGTTGCTTACTGGCCGAGAAACG(+CAGTGT)GTACCAATCTTTCAGCTCTGTGCTTGTCGGC NGG 10 8 17 8 24 0 100 5 10
    240605111454_f6xi AAGAAGCCGACAAGCACAGAGCTGAAAGATTG(+CAGTGT)GTACCGTTTCTCGGCCAGTAAGCAACAGAAC NGG 10 8 17 8 24 0 100 5 10