Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605111610_vzt8 CTTCATTGGCATGGCAACAGGTAGGCATATGG(+CAGTGT)GATCCTCCTAGTGGTTAGTACAATCACACCA NGG 10 8 17 8 24 0 100 5 10
    240605111609_1xmw CACAGTGGTGTGATTGTACTAACCACTAGGAG(+CAGTGT)GATCCCATATGCCTACCTGTTGCCATGCCAA NGG 10 8 17 8 24 0 100 5 10
    240605111607_7kku ACATGGACCTTTGGAAACCTGTGGATGACATG(+CAGTGT)GATCCGTGAAGAGCCCGGGTCACTACCACTC NGG 10 8 17 8 24 0 100 5 10
    240605111606_8kh2 GAAATGAGTGGTAGTGACCCGGGCTCTTCACG(+CAGTGT)GATCCATGTCATCCACAGGTTTCCAAAGGTC NGG 10 8 17 8 24 0 100 5 10
    240605111604_2nwc TCAAATCTATACACAGTAGGGCTATGAAGTTG(+CAGTGT)GTACCTCAGAAGTTCCTCCCCTGCCTGTTGG NGG 10 8 17 8 24 0 100 5 10
    240605111603_xhxo AGGTCCCAACAGGCAGGGGAGGAACTTCTGAG(+CAGTGT)GTACCAACTTCATAGCCCTACTGTGTATAGA NGG 10 8 17 8 24 0 100 5 10
    240605111601_ogib GCTCCGAGGTCAGGTGGACTGTCAGGGCCTAG(+CAGTGT)GATCCACCAGTTTGCCCGAGTTTACATTAGC NGG 10 8 17 8 24 0 100 5 10
    240605111559_8zuk ATGTTGCTAATGTAAACTCGGGCAAACTGGTG(+CAGTGT)GATCCTAGGCCCTGACAGTCCACCTGACCTC NGG 10 8 17 8 24 0 100 5 10
    240605111557_qp59 CTCCTGAGCCAATAGGGGCTTCCAGACCCAAG(+CAGTGT)GATCCGTTTCCTTGATGACTAAGAAGCCCCA NGG 10 8 17 8 24 0 100 5 10
    240605111556_ynsv AAGGGTGGGGCTTCTTAGTCATCAAGGAAACG(+CAGTGT)GATCCTTGGGTCTGGAAGCCCCTATTGGCTC NGG 10 8 17 8 24 0 100 5 10