Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605111721_wym2 TTTCCTTTTTAAATCATCCTGTGTGCATTCAG(+CAGTGT)GATCCTGGTCTTCATACAGAGATGTTGACAC NGG 10 8 17 8 24 0 100 5 10
    240605111719_jfzu GAAGGGTGTCAACATCTCTGTATGAAGACCAG(+CAGTGT)GATCCTGAATGCACACAGGATGATTTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605111716_lidm TTTGGTCTGGACTATGGGCTCATCAGGCCCAG(+CAGTGT)GATCCCGGACCAAAGACTGAGATGACCCTTC NGG 10 8 17 8 24 0 100 5 10
    240605111714_i7jz TATTGGAAGGGTCATCTCAGTCTTTGGTCCGG(+CAGTGT)GATCCTGGGCCTGATGAGCCCATAGTCCAGA NGG 10 8 17 8 24 0 100 5 10
    240605111713_b530 ATGCATGCTCTCTGTCTTTTGCTACAAAAATG(+CAGTGT)GTACCCAGATCTGCCTACTGCTTTCCTGCCC NGG 10 8 17 8 24 0 100 5 10
    240605111711_0o85 GGGGAGGGCAGGAAAGCAGTAGGCAGATCTGG(+CAGTGT)GTACCATTTTTGTAGCAAAAGACAGAGAGCA NGG 10 8 17 8 24 0 100 5 10
    240605111710_1lr4 ATGCATGCTCTCTGTCTTTTGCTACAAAAATG(+CAGTGT)GTACCCAGATCTGCCTACTGCTTTCCTGCCC NGG 10 8 17 8 24 0 100 5 10
    240605111708_na7r GGGGAGGGCAGGAAAGCAGTAGGCAGATCTGG(+CAGTGT)GTACCATTTTTGTAGCAAAAGACAGAGAGCA NGG 10 8 17 8 24 0 100 5 10
    240605111706_okyp CAAGAGATTAAACTCCTGGCCAGAGAGTGGGG(+CAGTGT)GTACCATATGGGAAAGGGGGAGCTAAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240605111704_xbx7 ATGCCCTGGCTTAGCTCCCCCTTTCCCATATG(+CAGTGT)GTACCCCCACTCTCTGGCCAGGAGTTTAATC NGG 10 8 17 8 24 0 100 5 10