Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605111703_n4er GAGCTGACACGGTTCAAGGCCTGTGTGTCTAG(+CAGTGT)GTACCCCGAGGCGATGCCGAGGGCTGCCACC NGG 10 8 17 8 24 0 100 5 10
    240605111701_aroa TGGTTGGTGGCAGCCCTCGGCATCGCCTCGGG(+CAGTGT)GTACCTAGACACACAGGCCTTGAACCGTGTC NGG 10 8 17 8 24 0 100 5 10
    240605111659_92u1 GGTGCTTCCTTACTCCAGTTACTTCCTGCTGG(+CAGTGT)GTACCAGTTTCTCTCAGAGGAGGACGCTGTC NGG 10 8 17 8 24 0 100 5 10
    240605111658_m5ip AGAAGGACAGCGTCCTCCTCTGAGAGAAACTG(+CAGTGT)GTACCCAGCAGGAAGTAACTGGAGTAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605111656_892g TTTCCCACATAGCATTTTATGTGTGTTCTGGG(+CAGTGT)GATCCAAACTCTGGCCGTAAAGCTTGCATAG NGG 10 8 17 8 24 0 100 5 10
    240605111655_eu6j GCTTACTATGCAAGCTTTACGGCCAGAGTTTG(+CAGTGT)GATCCCCAGAACACACATAAAATGCTATGTG NGG 10 8 17 8 24 0 100 5 10
    240605111653_6jka GGGGTCAGCGTTTGGAAGCTGATTATAGGATG(+CAGTGT)GATCCCCGGATATGGCAGTTTCTAGATGTCC NGG 10 8 17 8 24 0 100 5 10
    240605111650_5b8t AGGATGGACATCTAGAAACTGCCATATCCGGG(+CAGTGT)GATCCATCCTATAATCAGCTTCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240605111649_8oki TTGTCATGAGTACTTTACTAAAATATTTCAAG(+CAGTGT)GATCCAAATAGCATCAACTGTTTTCAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240605111648_rvyt ATCTGCTTTTTGAAAACAGTTGATGCTATTTG(+CAGTGT)GATCCTTGAAATATTTTAGTAAAGTACTCAT NGG 10 8 17 8 24 0 100 5 10