Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605111628_7ga4 ATCCAAATGCCTAGTACAGGGAAGCCGTAGAG(+CAGTGT)GTACCATCTGCAGCTTATTTTAAAAGCCATC NGG 10 8 17 8 24 0 100 5 10
    240605111627_y9b8 CCACTGATGGCTTTTAAAATAAGCTGCAGATG(+CAGTGT)GTACCTCTACGGCTTCCCTGTACTAGGCATT NGG 10 8 17 8 24 0 100 5 10
    240605111625_iwz8 TGAGCGGCTGCCGCCCCACCCTGTGCTCTCCG(+CAGTGT)GATCCCACTAGCCACAGGCATCTCCAGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605111623_2ixy CGGGCCCTCCTGGAGATGCCTGTGGCTAGTGG(+CAGTGT)GATCCGGAGAGCACAGGGTGGGGCGGCAGCC NGG 10 8 17 8 24 0 100 5 10
    240605111622_hfpx GCACCCAGATAGGCAAGGTCACCTTGACACTG(+CAGTGT)GTACCATGGTAACCTTGGCTCTTTAGGGCTG NGG 10 8 17 8 24 0 100 5 10
    240605111620_9t49 TGCACCAGCCCTAAAGAGCCAAGGTTACCATG(+CAGTGT)GTACCAGTGTCAAGGTGACCTTGCCTATCTG NGG 10 8 17 8 24 0 100 5 10
    240605111619_umrp CTGTGCTACCACACCCAGCCTGAGTTTAAATG(+CAGTGT)GTACCCTCGGACCCAAGGCAGCTCTGTGCTA NGG 10 8 17 8 24 0 100 5 10
    240605111616_q8c3 TGTCTTAGCACAGAGCTGCCTTGGGTCCGAGG(+CAGTGT)GTACCATTTAAACTCAGGCTGGGTGTGGTAG NGG 10 8 17 8 24 0 100 5 10
    240605111614_xh7a CTGTGCTACCACACCCAGCCTGAGTTTAAATG(+CAGTGT)GTACCCTCGGACCCAAGGCAGCTCTGTGCTA NGG 10 8 17 8 24 0 100 5 10
    240605111612_dwy6 TGTCTTAGCACAGAGCTGCCTTGGGTCCGAGG(+CAGTGT)GTACCATTTAAACTCAGGCTGGGTGTGGTAG NGG 10 8 17 8 24 0 100 5 10