Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605111554_dmyy TCCAAGAGCCCTGCTCTGCCCCTCGTGGCATG(+CAGTGT)GATCCTTTCCCTGGTGTGGACTTAAGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605111553_p87r TCCCCAGAGACTTAAGTCCACACCAGGGAAAG(+CAGTGT)GATCCATGCCACGAGGGGCAGAGCAGGGCTC NGG 10 8 17 8 24 0 100 5 10
    240605111551_zx1z CAGGAGGCTGCTATGGGCTAAAATTTTCCTAG(+CAGTGT)GTACCACCTAGAGGAGATGGAGCCGACCGAG NGG 10 8 17 8 24 0 100 5 10
    240605111550_teu1 CTTCCCTCGGTCGGCTCCATCTCCTCTAGGTG(+CAGTGT)GTACCTAGGAAAATTTTAGCCCATAGCAGCC NGG 10 8 17 8 24 0 100 5 10
    240605111548_fdm8 CAGGAATGCCATCTTCTGCCTTGGTCTGGAAG(+CAGTGT)GTACCCATGGCTACTTGCATCATTTTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605111546_xium ACAAGGCTGGAAAATGATGCAAGTAGCCATGG(+CAGTGT)GTACCTTCCAGACCAAGGCAGAAGATGGCAT NGG 10 8 17 8 24 0 100 5 10
    240605111543_o7n9 AAAAAGACCTGTGGAGATCTCAGAATCCACCG(+CAGTGT)GTACCATACTGCAAGGGATACTGACCTTAAG NGG 10 8 17 8 24 0 100 5 10
    240605111542_rf00 AGGAACTTAAGGTCAGTATCCCTTGCAGTATG(+CAGTGT)GTACCGGTGGATTCTGAGATCTCCACAGGTC NGG 10 8 17 8 24 0 100 5 10
    240605111540_x8up AGCCGAGACCCCCTCCCCTGCAGTCAGTGAGG(+CAGTGT)GATCCGTGGAGGTCCTGAACAAGTGTTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605111538_2q53 GAAGAAGACAACACTTGTTCAGGACCTCCACG(+CAGTGT)GATCCCTCACTGACTGCAGGGGAGGGGGTCT NGG 10 8 17 8 24 0 100 5 10