Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605111452_6ilu TCGTCAGCAGCCTGTCTCATTGCTTCAGCTGG(+CAGTGT)GTACCAGTCCTTCTGTTCCCCTGGGAAACTG NGG 10 8 17 8 24 0 100 5 10
    240605111451_w9oa GAGTTCAGTTTCCCAGGGGAACAGAAGGACTG(+CAGTGT)GTACCCAGCTGAAGCAATGAGACAGGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605111449_uo33 TGCAATCCCAGGGCTGGGAGATGGAGCCAGAG(+CAGTGT)GATCCCTGGGACTTGCTGGCCAGCCAGGCTA NGG 10 8 17 8 24 0 100 5 10
    240605111446_bw7r TTAACTAGCCTGGCTGGCCAGCAAGTCCCAGG(+CAGTGT)GATCCTCTGGCTCCATCTCCCAGCCCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605111444_l84x GTGGTATTGCATTTAAATGGACAGGACACGGG(+CAGTGT)GTACCAAATCCTACAATGTGTCAGTTTGGAG NGG 10 8 17 8 24 0 100 5 10
    240605111443_7x9w AATTGCTCCAAACTGACACATTGTAGGATTTG(+CAGTGT)GTACCCCGTGTCCTGTCCATTTAAATGCAAT NGG 10 8 17 8 24 0 100 5 10
    240605111441_wz3g TGCTCTTTATTCTCAGGGCCCGCACACATCCG(+CAGTGT)GTACCTTCATAAATGCAGTGTCACTGAAGAA NGG 10 8 17 8 24 0 100 5 10
    240605111440_j957 TCCATTTCTTCAGTGACACTGCATTTATGAAG(+CAGTGT)GTACCGGATGTGTGCGGGCCCTGAGAATAAA NGG 10 8 17 8 24 0 100 5 10
    240605111438_06tp AGCACACTGCTATGTGTGGCCTTGATGCTGGG(+CAGTGT)GATCCTAACTCAGGTTCTCGTGCTTTACCGA NGG 10 8 17 8 24 0 100 5 10
    240605111436_p90u CCCAGTCGGTAAAGCACGAGAACCTGAGTTAG(+CAGTGT)GATCCCCAGCATCAAGGCCACACATAGCAGT NGG 10 8 17 8 24 0 100 5 10