Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605111347_9vhe AGGAATCGATTCATAGTCCTGGGTATCAAAGG(+CAGTGT)GATCCAGTCACCAGGAGCTCCTCTCTTACCA NGG 10 8 17 8 24 0 100 5 10
    240605111345_0mfd ATCCCTGGTAAGAGAGGAGCTCCTGGTGACTG(+CAGTGT)GATCCCTTTGATACCCAGGACTATGAATCGA NGG 10 8 17 8 24 0 100 5 10
    240605111344_buah GGGGACAAGGTCACCTGAACTTAGCTCTCAAG(+CAGTGT)GATCCACACAAACTCAAAGCCTTAGTGGTAG NGG 10 8 17 8 24 0 100 5 10
    240605111343_3y89 GGCCACTACCACTAAGGCTTTGAGTTTGTGTG(+CAGTGT)GATCCTTGAGAGCTAAGTTCAGGTGACCTTG NGG 10 8 17 8 24 0 100 5 10
    240605111342_fdfp ACACAGCAATGGGAATAGCCACCGAGTTTAAG(+CAGTGT)GTACCAGGTGGCAACAAACCAATAATAACTT NGG 10 8 17 8 24 0 100 5 10
    240605111340_blqz TAGTTAAGTTATTATTGGTTTGTTGCCACCTG(+CAGTGT)GTACCTTAAACTCGGTGGCTATTCCCATTGC NGG 10 8 17 8 24 0 100 5 10
    240605111338_i8qr GTGGGCATATGTGGGTGCCAGGCTGGAACAAG(+CAGTGT)GTACCACTTTTAGAGTGAGATGTGACATTGT NGG 10 8 17 8 24 0 100 5 10
    240605111337_r3tu ACTCAACAATGTCACATCTCACTCTAAAAGTG(+CAGTGT)GTACCTTGTTCCAGCCTGGCACCCACATATG NGG 10 8 17 8 24 0 100 5 10
    240605111335_10ah CAACTTAGGGGGTGAGCAGGTGTTGGTGATGG(+CAGTGT)GATCCCTGTGATGAGATGGGGGTGACACTGC NGG 10 8 17 8 24 0 100 5 10
    240605111334_72lx AATGTGCAGTGTCACCCCCATCTCATCACAGG(+CAGTGT)GATCCCATCACCAACACCTGCTCACCCCCTA NGG 10 8 17 8 24 0 100 5 10