Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605111646_7cpq CAGGGGGCAAATTGGAAGCTGAGATCGACTTG(+CAGTGT)GATCCTGGGAAGGATGAAGATGACCTTCTGC NGG 10 8 17 8 24 0 100 5 10
    240605111644_u33q CTCAAGCAGAAGGTCATCTTCATCCTTCCCAG(+CAGTGT)GATCCAAGTCGATCTCAGCTTCCAATTTGCC NGG 10 8 17 8 24 0 100 5 10
    240605111642_nblk AGAACACAGGGGGTCCCCAACCCACTCTCAGG(+CAGTGT)GATCCACCTGGTGTCTTGGGTCTGTCAGGGA NGG 10 8 17 8 24 0 100 5 10
    240605111640_3aok TCTGCTCCCTGACAGACCCAAGACACCAGGTG(+CAGTGT)GATCCCTGAGAGTGGGTTGGGGACCCCCTGT NGG 10 8 17 8 24 0 100 5 10
    240605111638_k8l0 TTCCCCCACCTAACCCACTTTTGAAACCAAAG(+CAGTGT)GTACCTTGCCTCAGCGACACAAAGAGATGGG NGG 10 8 17 8 24 0 100 5 10
    240605111637_jr8v GATCTCCCATCTCTTTGTGTCGCTGAGGCAAG(+CAGTGT)GTACCTTTGGTTTCAAAAGTGGGTTAGGTGG NGG 10 8 17 8 24 0 100 5 10
    240605111635_wajs TCTCTGTCCTGGTCCCGCCCCCAACCATGGTG(+CAGTGT)GATCCTGGAGGACCTCGTACCCCTCCCCTCC NGG 10 8 17 8 24 0 100 5 10
    240605111633_mijv GTGGGGGAGGGGAGGGGTACGAGGTCCTCCAG(+CAGTGT)GATCCACCATGGTTGGGGGCGGGACCAGGAC NGG 10 8 17 8 24 0 100 5 10
    240605111631_egdq CTGCCTATAAATACCTCTCCAGGGGCTGGGAG(+CAGTGT)GTACCCTCTGTTCCATCCACCACACTGCTCA NGG 10 8 17 8 24 0 100 5 10
    240605111629_u0py GGCAATGAGCAGTGTGGTGGATGGAACAGAGG(+CAGTGT)GTACCTCCCAGCCCCTGGAGAGGTATTTATA NGG 10 8 17 8 24 0 100 5 10