Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605111739_0rwv CAACACCATTTACCTAGAAAGGACCAAGTTTG(+CAGTGT)GATCCTGAAGCCCAGTGGAGCACCATTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605111737_qfyc CAGGCAGAGAATGGTGCTCCACTGGGCTTCAG(+CAGTGT)GATCCAAACTTGGTCCTTTCTAGGTAAATGG NGG 10 8 17 8 24 0 100 5 10
    240605111736_e8pv TGAGAAGAGCCTCTGACAGGCATCAGAGCTGG(+CAGTGT)GATCCTGACCAAGGGACACTCAGGCTGCCAC NGG 10 8 17 8 24 0 100 5 10
    240605111734_0m53 AGCTGGTGGCAGCCTGAGTGTCCCTTGGTCAG(+CAGTGT)GATCCCAGCTCTGATGCCTGTCAGAGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240605111732_8i6z ACAGATAGTGTATGAAATACACAATTTCCCAG(+CAGTGT)GTACCAAGGGCCAATAGCAAAAAGGGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605111729_o6q5 CTTTTAGAGACCCTTTTTGCTATTGGCCCTTG(+CAGTGT)GTACCTGGGAAATTGTGTATTTCATACACTA NGG 10 8 17 8 24 0 100 5 10
    240605111726_y52k TTGACTGTCTCTGCATAGTCTTGATACCCCAG(+CAGTGT)GATCCTGACATCCCCAGAAATTATACCCCGC NGG 10 8 17 8 24 0 100 5 10
    240605111725_2dqk GGGGGGCGGGGTATAATTTCTGGGGATGTCAG(+CAGTGT)GATCCTGGGGTATCAAGACTATGCAGAGACA NGG 10 8 17 8 24 0 100 5 10
    240605111723_2qxq TGCACACAGTGAGGAATAAACATTCTGGAAAG(+CAGTGT)GATCCTTGATTCTCCCACAAACTGTTCCTTG NGG 10 8 17 8 24 0 100 5 10
    240605111722_bqpb TACTCCAAGGAACAGTTTGTGGGAGAATCAAG(+CAGTGT)GATCCTTTCCAGAATGTTTATTCCTCACTGT NGG 10 8 17 8 24 0 100 5 10