Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605111217_pgzh GGCTATTCCTTGTCCCTACACTGCTCTCTTGG(+CAGTGT)GTACCTCTGGGGCACCCTGGCAGGAATGAGA NGG 10 8 17 8 24 0 100 5 10
    240605111215_9lkf AAAGTTCTCATTCCTGCCAGGGTGCCCCAGAG(+CAGTGT)GTACCCAAGAGAGCAGTGTAGGGACAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605111213_lgpw GTGGTACAGGGTCCTGGTCTGCTGTGTGTGGG(+CAGTGT)GATCCTGGCTGCAGAGCTTCTTCTGAGTAGA NGG 10 8 17 8 24 0 100 5 10
    240605111211_i01b TACCTTCTACTCAGAAGAAGCTCTGCAGCCAG(+CAGTGT)GATCCCCACACACAGCAGACCAGGACCCTGT NGG 10 8 17 8 24 0 100 5 10
    240605111210_jyho CAGCAGTTTTAAAGTCTAACACATTTTAAACG(+CAGTGT)GATCCAAAGTCTTCTTTCCTCATCCGTCACT NGG 10 8 17 8 24 0 100 5 10
    240605111208_1d58 TGCTAAGTGACGGATGAGGAAAGAAGACTTTG(+CAGTGT)GATCCGTTTAAAATGTGTTAGACTTTAAAAC NGG 10 8 17 8 24 0 100 5 10
    240605111207_cgam GGCACTGGAGGAAACTATTTCTCCCTCTCAAG(+CAGTGT)GATCCGGGCGTGCTCTGCACCCGGCCTTGGT NGG 10 8 17 8 24 0 100 5 10
    240605111204_pm9g AGGCCACCAAGGCCGGGTGCAGAGCACGCCCG(+CAGTGT)GATCCTTGAGAGGGAGAAATAGTTTCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240605111202_ezec TGACAGGCTCTGGAGCTGCCTCCTAGGCTGGG(+CAGTGT)GTACCCCTAGGCCCTGGCAACCCACGTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605111200_p5ma TGTAAAACAACGTGGGTTGCCAGGGCCTAGGG(+CAGTGT)GTACCCCAGCCTAGGAGGCAGCTCCAGAGCC NGG 10 8 17 8 24 0 100 5 10