Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613163215_02lx GTACGGTTTACAGATAGCACTGCATGTGGGGG(+AGGACA)GTACCGTGTGTACATCACTCTTTAGGAGGGT NGG 10 8 17 8 24 0 100 5 10
    240613163213_5nkh GACTGGCAGGTATTGTAGGTGTTGTGACTGTG(+AGGACA)GTACCTCCTAGGAATGCTGTTGGAGACAGAA NGG 10 8 17 8 24 0 100 5 10
    240613163212_gjuh TGGGATTCTGTCTCCAACAGCATTCCTAGGAG(+AGGACA)GTACCACAGTCACAACACCTACAATACCTGC NGG 10 8 17 8 24 0 100 5 10
    240613163210_a1wj CAAGCTGGACTGAGCCTGGGGACAGGGTTAGG(+AGGACA)GTACCTGCCCCTGGAAAGGCTGCTGCAAGGA NGG 10 8 17 8 24 0 100 5 10
    240613163209_lwiw TCAGATCCTTGCAGCAGCCTTTCCAGGGGCAG(+AGGACA)GTACCCTAACCCTGTCCCCAGGCTCAGTCCA NGG 10 8 17 8 24 0 100 5 10
    240613163207_psj5 AGAGCAATGTCTGATTTCACAACTTTTTCACG(+AGGACA)GATCCCTGAGACCACGATTAAAATCAGGTTT NGG 10 8 17 8 24 0 100 5 10
    240613163206_yait TTTTAAAACCTGATTTTAATCGTGGTCTCAGG(+AGGACA)GATCCGTGAAAAAGTTGTGAAATCAGACATT NGG 10 8 17 8 24 0 100 5 10
    240613163204_qwxa AGTTCTGTGGCTGTGCTCATCTGTGTCATGGG(+AGGACA)GATCCCCCACAACCTTTGACTTTCTTTGGAG NGG 10 8 17 8 24 0 100 5 10
    240613163201_zoo1 AAATGCTCCAAAGAAAGTCAAAGGTTGTGGGG(+AGGACA)GATCCCCATGACACAGATGAGCACAGCCACA NGG 10 8 17 8 24 0 100 5 10
    240613163200_yojy ATGGACCAAGGGTTTCATGGGGCAGAAGGAGG(+AGGACA)GTACCCAGAATTCCCAGCCCTCATCTGGTCC NGG 10 8 17 8 24 0 100 5 10