Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613163141_sl4c CCCACGGGGACTGAGGTCTGTTTAGCTCTGAG(+AGGACA)GATCCCAGTCAAAGGGCAAGCAGTAGAAGAG NGG 10 8 17 8 24 0 100 5 10
    240613163139_ez61 TTGCTGGGGTTAATGGCAAAGGCCTCAGGAGG(+AGGACA)GATCCTACAATCACCCCAGTTCCAGTGTGTA NGG 10 8 17 8 24 0 100 5 10
    240613163137_bmqv GGAAGTACACACTGGAACTGGGGTGATTGTAG(+AGGACA)GATCCCTCCTGAGGCCTTTGCCATTAACCCC NGG 10 8 17 8 24 0 100 5 10
    240613163136_ejxh CCCAACAAGCCCAGGCCCCGTAACCTTGGGTG(+AGGACA)GTACCCAGCACCTTTGCTGCCCACACCCTTG NGG 10 8 17 8 24 0 100 5 10
    240613163134_3i3g GGACCCAAGGGTGTGGGCAGCAAAGGTGCTGG(+AGGACA)GTACCACCCAAGGTTACGGGGCCTGGGCTTG NGG 10 8 17 8 24 0 100 5 10
    240613163132_a4e9 GGTTTTGTAATTTTTTTGGCATGTACGTGCAG(+AGGACA)GTACCTATTATTATTATTATTTCTGTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240613163131_7b6l AGGAATTTAAACAGAAATAATAATAATAATAG(+AGGACA)GTACCTGCACGTACATGCCAAAAAAATTACA NGG 10 8 17 8 24 0 100 5 10
    240613163130_ytbx GCTAGAACAAACCCAGAGGCTTAAACAACAGG(+AGGACA)GATCCGGTCCCAGGCCACGTGCACAAGATGC NGG 10 8 17 8 24 0 100 5 10
    240613163128_kh7g GAATAGCATCTTGTGCACGTGGCCTGGGACCG(+AGGACA)GATCCCTGTTGTTTAAGCCTCTGGGTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240613163126_z85v TCTGAAAAGCTTCAGGATCAGCCTAATCTCCG(+AGGACA)GATCCTTATTAAGTGCATCTGCGAGGTCCCG NGG 10 8 17 8 24 0 100 5 10