Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613163124_3k6d GTAGCCGGGACCTCGCAGATGCACTTAATAAG(+AGGACA)GATCCGGAGATTAGGCTGATCCTGAAGCTTT NGG 10 8 17 8 24 0 100 5 10
    240613163123_2kfe GTGTGCTTTCACAGAGCGCTCCTCCCTCGTTG(+AGGACA)GTACCCTGGCCACTTCCTCTGCACAGTACAG NGG 10 8 17 8 24 0 100 5 10
    240613163121_gwkz GAGCTCTGTACTGTGCAGAGGAAGTGGCCAGG(+AGGACA)GTACCAACGAGGGAGGAGCGCTCTGTGAAAG NGG 10 8 17 8 24 0 100 5 10
    240613163117_6bcp AGTCAGTAAGAATGACCCTGCTGTGCCCCAGG(+AGGACA)GTACCCATTCCCCACAGCATGCTTATGCCAT NGG 10 8 17 8 24 0 100 5 10
    240613163116_6k7v GTCTCATGGCATAAGCATGCTGTGGGGAATGG(+AGGACA)GTACCCTGGGGCACAGCAGGGTCATTCTTAC NGG 10 8 17 8 24 0 100 5 10
    240613163115_5t0f CTTGCATGTGGAGACCAGGGGTTGACATCAAG(+AGGACA)GATCCTCCTTAATCATTTATCCACTCACCAT NGG 10 8 17 8 24 0 100 5 10
    240613163113_fe3l TTTAAATGGTGAGTGGATAAATGATTAAGGAG(+AGGACA)GATCCTTGATGTCAACCCCTGGTCTCCACAT NGG 10 8 17 8 24 0 100 5 10
    240613163111_u6y1 GGCACTGACCTGCCCCATTTCCTGATCCTTGG(+AGGACA)GATCCTAGAAACGCATACAAGGAGGGACAGT NGG 10 8 17 8 24 0 100 5 10
    240613163109_hcvj AGGCCACTGTCCCTCCTTGTATGCGTTTCTAG(+AGGACA)GATCCCAAGGATCAGGAAATGGGGCAGGTCA NGG 10 8 17 8 24 0 100 5 10
    240613163108_q26v TCCTGTTCAAATGTACCTCAGTGGAGAGAGAG(+AGGACA)GATCCTCGCTTCTACACTGTGTGGCCTGACG NGG 10 8 17 8 24 0 100 5 10