Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613163158_cafi GGCTGGGACCAGATGAGGGCTGGGAATTCTGG(+AGGACA)GTACCCTCCTTCTGCCCCATGAAACCCTTGG NGG 10 8 17 8 24 0 100 5 10
    240613163156_flfn CCATTGCTTCCCCTTATAAGATGTCCCTGCAG(+AGGACA)GTACCAGAGCCAGTGACAGCACACACACAGG NGG 10 8 17 8 24 0 100 5 10
    240613163155_7c0w AGGGTCCTGTGTGTGTGCTGTCACTGGCTCTG(+AGGACA)GTACCTGCAGGGACATCTTATAAGGGGAAGC NGG 10 8 17 8 24 0 100 5 10
    240613163153_krjp GGCGTACCCAGGCCAGCACCAGAGGGCGGCAG(+AGGACA)GTACCCCAGGGAGTGGCTAGGGAGGTTGGTC NGG 10 8 17 8 24 0 100 5 10
    240613163151_6rli CCTTCGACCAACCTCCCTAGCCACTCCCTGGG(+AGGACA)GTACCTGCCGCCCTCTGGTGCTGGCCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240613163149_xt7x CCCTGACGGTAGCACCAGGAGATCATGGATAG(+AGGACA)GATCCCAGGTTCAAGGTCAGGGGCTCAGCCT NGG 10 8 17 8 24 0 100 5 10
    240613163147_hf0t GAGTGAGGCTGAGCCCCTGACCTTGAACCTGG(+AGGACA)GATCCTATCCATGATCTCCTGGTGCTACCGT NGG 10 8 17 8 24 0 100 5 10
    240613163146_dlmn AGTGTACAGCCAGGATAGGCCAAAACCTTTCG(+AGGACA)GATCCCAGGATGAGACCAAGTCTCCATCAGG NGG 10 8 17 8 24 0 100 5 10
    240613163144_h5q7 TAGGACCTGATGGAGACTTGGTCTCATCCTGG(+AGGACA)GATCCGAAAGGTTTTGGCCTATCCTGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240613163142_c8xt AGCCTCTCTTCTACTGCTTGCCCTTTGACTGG(+AGGACA)GATCCTCAGAGCTAAACAGACCTCAGTCCCC NGG 10 8 17 8 24 0 100 5 10