Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605011109_g2ra TAAGAATTTACATGCACATCGCTTGACATTCG(+ACTCAC)GTACCCAGAGAATTCTTTATGTGCCTTTCAA NGG 10 8 17 8 24 0 100 5 10
    240605011108_2toc CTCTCTTGAAAGGCACATAAAGAATTCTCTGG(+ACTCAC)GTACCGAATGTCAAGCGATGTGCATGTAAAT NGG 10 8 17 8 24 0 100 5 10
    240605011105_k4nh TGGCTGGCACAGCCCGGTCTCAGGCCCCCTGG(+ACTCAC)GATCCAGACCTCCTCCTTGCACTGAGAATGC NGG 10 8 17 8 24 0 100 5 10
    240605011104_qw1c AAAGTGCATTCTCAGTGCAAGGAGGAGGTCTG(+ACTCAC)GATCCCAGGGGGCCTGAGACCGGGCTGTGCC NGG 10 8 17 8 24 0 100 5 10
    240605011102_ibgr TGTGTTTCCCTTTAGTCTCCGCCTATACTTGG(+ACTCAC)GATCCTTTCTCACTGCTGGCCATTCAGTGAG NGG 10 8 17 8 24 0 100 5 10
    240605011101_q51w CCTTCCTCACTGAATGGCCAGCAGTGAGAAAG(+ACTCAC)GATCCCAAGTATAGGCGGAGACTAAAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605011059_fwzy TGTGACTGATTATATGGTCAATTTTGGAGAAG(+ACTCAC)GTACCATGAGATGCTGAGAAGAAGGTATATT NGG 10 8 17 8 24 0 100 5 10
    240605011058_l177 AAAAGAATATACCTTCTTCTCAGCATCTCATG(+ACTCAC)GTACCTTCTCCAAAATTGACCATATAATCAG NGG 10 8 17 8 24 0 100 5 10
    240605011057_u5qi GCCTTAGTTGCTATTTTACAGTACTGTTAAAG(+ACTCAC)GATCCATTGGCATGGGATGGGAAGCAGCAGG NGG 10 8 17 8 24 0 100 5 10
    240605011055_s3c7 TGGGCCCTGCTGCTTCCCATCCCATGCCAATG(+ACTCAC)GATCCTTTAACAGTACTGTAAAATAGCAACT NGG 10 8 17 8 24 0 100 5 10