Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605011006_fel4 TTTGTGTTAAAATCTGTGAAGTATTAAGACTG(+ACTCAC)GTACCAGATTTCACAAGAAAAATGTAATTTC NGG 10 8 17 8 24 0 100 5 10
    240605011005_rc9d AGATGGAAATTACATTTTTCTTGTGAAATCTG(+ACTCAC)GTACCAGTCTTAATACTTCACAGATTTTAAC NGG 10 8 17 8 24 0 100 5 10
    240605011003_ntd2 TAATGTACAGCGAGGCCCAGAGGTGTACAGTG(+ACTCAC)GATCCACAGCGGGGGGAAAAAAGGCAGAGTT NGG 10 8 17 8 24 0 100 5 10
    240605011002_o32w CCTAGAACTCTGCCTTTTTTCCCCCCGCTGTG(+ACTCAC)GATCCACTGTACACCTCTGGGCCTCGCTGTA NGG 10 8 17 8 24 0 100 5 10
    240605011000_lglm GGAGCAGGGACCTGATCTCCTATCTGTCCTGG(+ACTCAC)GATCCATTTTCAGAGGGGCAGTTTTATTTGG NGG 10 8 17 8 24 0 100 5 10
    240605010958_a5v7 CCAGGCCAAATAAAACTGCCCCTCTGAAAATG(+ACTCAC)GATCCCAGGACAGATAGGAGATCAGGTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605010957_mpo5 GTTAAGGAACTGGCCTTGGATTTTCTTTCTCG(+ACTCAC)GTACCCATCTCTTTTTACTTACACTCTACAG NGG 10 8 17 8 24 0 100 5 10
    240605010956_a32q AATATCTGTAGAGTGTAAGTAAAAAGAGATGG(+ACTCAC)GTACCGAGAAAGAAAATCCAAGGCCAGTTCC NGG 10 8 17 8 24 0 100 5 10
    240605010954_0xna CGGGAGGACCTAGTTCTCCTCTGATCTCTGAG(+ACTCAC)GTACCTGCACTTGTGCGTGCCAGACCCATGC NGG 10 8 17 8 24 0 100 5 10
    240605010953_mpne TCTGTGCATGGGTCTGGCACGCACAAGTGCAG(+ACTCAC)GTACCTCAGAGATCAGAGGAGAACTAGGTCC NGG 10 8 17 8 24 0 100 5 10