Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605011023_rme3 CAGCACAGTAGAGTCACCGAGGGGTATGGTAG(+ACTCAC)GTACCAGAGGAACTATTCAGTCCTCATGGAA NGG 10 8 17 8 24 0 100 5 10
    240605011021_4zhs GTCTGTTCCATGAGGACTGAATAGTTCCTCTG(+ACTCAC)GTACCTACCATACCCCTCGGTGACTCTACTG NGG 10 8 17 8 24 0 100 5 10
    240605011019_p3xp CTCTCAATCGGGGCTGGAAAAGAACAAAGTGG(+ACTCAC)GATCCCACACTTCTCCTGCACCCTCCAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605011018_xy8i GCAACCTGCTGGAGGGTGCAGGAGAAGTGTGG(+ACTCAC)GATCCCACTTTGTTCTTTTCCAGCCCCGATT NGG 10 8 17 8 24 0 100 5 10
    240605011017_3td3 GCCATCCATGGAATGAGTTTATGTGTACAAAG(+ACTCAC)GATCCAGAAAAGGGGACATTTTAAAAAAATC NGG 10 8 17 8 24 0 100 5 10
    240605011015_3roo ACAGTGATTTTTTTAAAATGTCCCCTTTTCTG(+ACTCAC)GATCCTTTGTACACATAAACTCATTCCATGG NGG 10 8 17 8 24 0 100 5 10
    240605011014_kczi ACTTTGGCAGGACTCTTCCCAGAGGGTCACAG(+ACTCAC)GATCCAAGGCCAGGTCTGTAAATAGCAGTGT NGG 10 8 17 8 24 0 100 5 10
    240605011012_va5q TATACACACTGCTATTTACAGACCTGGCCTTG(+ACTCAC)GATCCTGTGACCCTCTGGGAAGAGTCCTGCC NGG 10 8 17 8 24 0 100 5 10
    240605011009_ktvr GTACTGGGGAGGAAACTAATCCAACTTCCCAG(+ACTCAC)GATCCTCTACCACCCCCATGTTCAGCTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605011008_gk7k GGGCAAGAGAGCTGAACATGGGGGTGGTAGAG(+ACTCAC)GATCCTGGGAAGTTGGATTAGTTTCCTCCCC NGG 10 8 17 8 24 0 100 5 10