Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605011038_6qbr TGTGGGGCATTTCTTATGCCCTGCCCCCAAGG(+ACTCAC)GATCCAAGTCACCACATTGCCAAAGAATCCA NGG 10 8 17 8 24 0 100 5 10
    240605011037_8x5q AAGCCTGGATTCTTTGGCAATGTGGTGACTTG(+ACTCAC)GATCCCTTGGGGGCAGGGCATAAGAAATGCC NGG 10 8 17 8 24 0 100 5 10
    240605011036_lgw9 TGGCCAACAAGGTGGCTGACCCAATCGGTGAG(+ACTCAC)GATCCAAGAGATGGAGGGAGAGCACGAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605011034_hbrm GTTTTACTTTCGTGCTCTCCCTCCATCTCTTG(+ACTCAC)GATCCTCACCGATTGGGTCAGCCACCTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605011033_p80y TTAAACCATTTTATGAGTACCATTCTGCAAAG(+ACTCAC)GATCCCATAGTGGTGCACAGCCTTAAGAAGC NGG 10 8 17 8 24 0 100 5 10
    240605011031_3ax5 GCCTGGCTTCTTAAGGCTGTGCACCACTATGG(+ACTCAC)GATCCTTTGCAGAATGGTACTCATAAAATGG NGG 10 8 17 8 24 0 100 5 10
    240605011030_8fvu CAGTTACTAAGGCACATGACCGTGGTGAGGAG(+ACTCAC)GATCCTCAGGACCTGCCGGAGCTGCCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605011028_dnsm GGTGGCTGCAGGCAGCTCCGGCAGGTCCTGAG(+ACTCAC)GATCCTCCTCACCACGGTCATGTGCCTTAGT NGG 10 8 17 8 24 0 100 5 10
    240605011027_j9jo CTGTGTTTAGGTGTCACGTGTGAGATAATGTG(+ACTCAC)GTACCAGGAAAGGAGCTATGTACTGAGGCTT NGG 10 8 17 8 24 0 100 5 10
    240605011025_oqrn GAAACAAGCCTCAGTACATAGCTCCTTTCCTG(+ACTCAC)GTACCACATTATCTCACACGTGACACCTAAA NGG 10 8 17 8 24 0 100 5 10