Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605004209_ciz3 AGGCAAAGAAAGGAGTGTCATTATTTTGAATG(+ACTCAC)GTACCAATTGTATGGTCTGTTGTACTTGAGT NGG 10 8 17 8 24 0 100 5 10
    240605004208_rtqa CTGGCAGAGAAATTAGAACTACCTTTTTCTAG(+ACTCAC)GATCCTCAGAAGCCAAACCACTTTGGTTATA NGG 10 8 17 8 24 0 100 5 10
    240605004206_nslu GAGCATATAACCAAAGTGGTTTGGCTTCTGAG(+ACTCAC)GATCCTAGAAAAAGGTAGTTCTAATTTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605004204_jit2 TGGTGAGTAAGCCAGCGTCGGCTCAGAGCCTG(+ACTCAC)GATCCCAGTTACTTCCACATCTGCGGGGCCA NGG 10 8 17 8 24 0 100 5 10
    240605004203_0esh AACTGTGGCCCCGCAGATGTGGAAGTAACTGG(+ACTCAC)GATCCAGGCTCTGAGCCGACGCTGGCTTACT NGG 10 8 17 8 24 0 100 5 10
    240605004201_xke3 GTTATGGCCCAGCTGCTGCCACCTTGTCCTTG(+ACTCAC)GTACCATAAGATATCATGGAACGCCCAGTTC NGG 10 8 17 8 24 0 100 5 10
    240605004159_r4ut GTGTGGAACTGGGCGTTCCATGATATCTTATG(+ACTCAC)GTACCAAGGACAAGGTGGCAGCAGCTGGGCC NGG 10 8 17 8 24 0 100 5 10
    240605004158_wlcf AATTTTACCATCAATACAGGCTCAGTGATAGG(+ACTCAC)GTACCCTTTTCCAGGAACTGGGTTTAATTGC NGG 10 8 17 8 24 0 100 5 10
    240605004156_3664 TGCTGGCAATTAAACCCAGTTCCTGGAAAAGG(+ACTCAC)GTACCCTATCACTGAGCCTGTATTGATGGTA NGG 10 8 17 8 24 0 100 5 10
    240605004155_is6d TGATAGAAAAGTCAGAAATTCCATCAGTGTGG(+ACTCAC)GTACCAGGAAATGTGGTTGGACCAGCCCTGG NGG 10 8 17 8 24 0 100 5 10