Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605004153_z8fc GGCATCCAGGGCTGGTCCAACCACATTTCCTG(+ACTCAC)GTACCCACACTGATGGAATTTCTGACTTTTC NGG 10 8 17 8 24 0 100 5 10
    240605004151_72kk GTGTTGGGAGCTTAGGACAGAATGTCCTCAGG(+ACTCAC)GATCCTGGGCTCTATGACAGAGCTTGCAGGT NGG 10 8 17 8 24 0 100 5 10
    240605004149_6ei0 AAACCACCTGCAAGCTCTGTCATAGAGCCCAG(+ACTCAC)GATCCCTGAGGACATTCTGTCCTAAGCTCCC NGG 10 8 17 8 24 0 100 5 10
    240605004147_m4w0 GATGCATATGTGATGACTTTCTTAGCAGTTGG(+ACTCAC)GATCCACTCATATGTTTCAAAACTTTACCTA NGG 10 8 17 8 24 0 100 5 10
    240605004146_4t4b ACTCCTAGGTAAAGTTTTGAAACATATGAGTG(+ACTCAC)GATCCCAACTGCTAAGAAAGTCATCACATAT NGG 10 8 17 8 24 0 100 5 10
    240605004144_w3ux AAATAAGACCAGGACCACAGGGAATCTACCAG(+ACTCAC)GATCCTTCCCACGCCCTTCCTGAAGCATCAA NGG 10 8 17 8 24 0 100 5 10
    240605004143_mfnf GATCGTTGATGCTTCAGGAAGGGCGTGGGAAG(+ACTCAC)GATCCTGGTAGATTCCCTGTGGTCCTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240605004141_pxag CCTCGACATGGTCTCCTGGGGTGGTTTCTAAG(+ACTCAC)GTACCTTCCTGTTTAATACTACATAACCTCG NGG 10 8 17 8 24 0 100 5 10
    240605004140_ha5u CTTTCCGAGGTTATGTAGTATTAAACAGGAAG(+ACTCAC)GTACCTTAGAAACCACCCCAGGAGACCATGT NGG 10 8 17 8 24 0 100 5 10
    240605004138_2lwd AAATAGGCAGTCACCCGAAAAGACTTTTCCTG(+ACTCAC)GATCCGTTTGCCTCAGCTATTTGTGGGAGGC NGG 10 8 17 8 24 0 100 5 10