Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605004136_k5h6 ACCAAGCCTCCCACAAATAGCTGAGGCAAACG(+ACTCAC)GATCCAGGAAAAGTCTTTTCGGGTGACTGCC NGG 10 8 17 8 24 0 100 5 10
    240605004135_o77w ATCGGTAGGCACGCTGCTCCTCTTGCTGCAGG(+ACTCAC)GTACCCCAGGGGAGGTGTTCTTGGCTACCAG NGG 10 8 17 8 24 0 100 5 10
    240605004132_0hkg GCCTCCTGGTAGCCAAGAACACCTCCCCTGGG(+ACTCAC)GTACCCTGCAGCAAGAGGAGCAGCGTGCCTA NGG 10 8 17 8 24 0 100 5 10
    240605004131_wd0v GCTCACAACTGTGAATAACACCAACCTCACTG(+ACTCAC)GATCCAACAGCCTCCTTCCCACTTCTTGGTC NGG 10 8 17 8 24 0 100 5 10
    240605004130_g30a TGGGTGACCAAGAAGTGGGAAGGAGGCTGTTG(+ACTCAC)GATCCAGTGAGGTTGGTGTTATTCACAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605004129_6gwn ACCAGACCCGAGTCTGCGGCCTCCCTGAGAAG(+ACTCAC)GTACCGCAAGGAGAATCAGCACCCTGGCTCG NGG 10 8 17 8 24 0 100 5 10
    240605004127_odt6 CACTCCGAGCCAGGGTGCTGATTCTCCTTGCG(+ACTCAC)GTACCTTCTCAGGGAGGCCGCAGACTCGGGT NGG 10 8 17 8 24 0 100 5 10
    240605004125_7kaq TCAATTCTTTCCATCTTTAAGTAGGTTTTGGG(+ACTCAC)GATCCTACTCAGGTCACCAGGCTTGTATTAC NGG 10 8 17 8 24 0 100 5 10
    240605004124_yu12 TACTTGTAATACAAGCCTGGTGACCTGAGTAG(+ACTCAC)GATCCCCAAAACCTACTTAAAGATGGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605004122_2rul GTCAGAGCTGCTACATGGGGAAGAGGAAGGAG(+ACTCAC)GTACCTGACGGCTCGGAAGCCAGAGGATCAG NGG 10 8 17 8 24 0 100 5 10