Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605004121_4zl3 ACAAACTGATCCTCTGGCTTCCGAGCCGTCAG(+ACTCAC)GTACCTCCTTCCTCTTCCCCATGTAGCAGCT NGG 10 8 17 8 24 0 100 5 10
    240605004118_eyph GTTTCTCCCCCCTTCTTACTCCCTCTCCCGTG(+ACTCAC)GATCCCAGAGCCTCATGCATGCTATGCAAAA NGG 10 8 17 8 24 0 100 5 10
    240605004116_vad2 AAGTGTTTTGCATAGCATGCATGAGGCTCTGG(+ACTCAC)GATCCACGGGAGAGGGAGTAAGAAGGGGGGA NGG 10 8 17 8 24 0 100 5 10
    240605004114_bf4s TGCGACACCCGGGGCACCAGTTCCATTACCAG(+ACTCAC)GATCCCTGTGAGAACATTTGCATCTCTTATA NGG 10 8 17 8 24 0 100 5 10
    240605004113_m2a8 CCTGTTATAAGAGATGCAAATGTTCTCACAGG(+ACTCAC)GATCCTGGTAATGGAACTGGTGCCCCGGGTG NGG 10 8 17 8 24 0 100 5 10
    240605004111_uflw CTAGTTAACTCACTCCCATAATGTTCCTTTGG(+ACTCAC)GTACCAGCTGTAAGCCAACACCACTCACAAA NGG 10 8 17 8 24 0 100 5 10
    240605004110_2gtt TAATTTTTGTGAGTGGTGTTGGCTTACAGCTG(+ACTCAC)GTACCCAAAGGAACATTATGGGAGTGAGTTA NGG 10 8 17 8 24 0 100 5 10
    240605004107_rls7 GAAAGGGTAGATGGTGCCAGTTATTTGCCCTG(+ACTCAC)GTACCTTTGGGCCCAGGAAGTTCATCACGGT NGG 10 8 17 8 24 0 100 5 10
    240605004105_pn9c TGCACACCGTGATGAACTTCCTGGGCCCAAAG(+ACTCAC)GTACCAGGGCAAATAACTGGCACCATCTACC NGG 10 8 17 8 24 0 100 5 10
    240605004104_8hqb TCTCTTCCCCTAGGATGTTTCTCAATTGAGTG(+ACTCAC)GATCCCAGCAGTTTACATTTGCCACAAGTTA NGG 10 8 17 8 24 0 100 5 10