Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605004015_vlij GGACACCATGGTCCAGCCCCACTTCCATACAG(+ACTCAC)GATCCTCTGGAATTCCAGAAGGGAGCTCTAG NGG 10 8 17 8 24 0 100 5 10
    240605004013_29je CCAACCGCTACAGCTATTAGTGAGGAGGCAAG(+ACTCAC)GATCCCCTCAGAGTGCCACAATCGGAAGCTA NGG 10 8 17 8 24 0 100 5 10
    240605004011_kfon TAGTTTAGCTTCCGATTGTGGCACTCTGAGGG(+ACTCAC)GATCCTTGCCTCCTCACTAATAGCTGTAGCG NGG 10 8 17 8 24 0 100 5 10
    240605004010_r28m CCAACCGCTACAGCTATTAGTGAGGAGGCAAG(+ACTCAC)GATCCCCTCAGAGTGCCACAATCGGAAGCTA NGG 10 8 17 8 24 0 100 5 10
    240605004008_4afp TAGTTTAGCTTCCGATTGTGGCACTCTGAGGG(+ACTCAC)GATCCTTGCCTCCTCACTAATAGCTGTAGCG NGG 10 8 17 8 24 0 100 5 10
    240605004006_8899 GGTGACCCTTGGCCACCAGACAATACAGGGAG(+ACTCAC)GTACCTGGGGAGCAGCTAGGAAATCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240605004003_5zei AGGGGGCCTGTGATTTCCTAGCTGCTCCCCAG(+ACTCAC)GTACCTCCCTGTATTGTCTGGTGGCCAAGGG NGG 10 8 17 8 24 0 100 5 10
    240605004001_z0tk GATGGGTTGGGAGACTCTCAAAATGTGCCAAG(+ACTCAC)GATCCCCTACGTTCTAGTCCAGGGAAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605003959_1940 GGCGTTTCCCTTCCCTGGACTAGAACGTAGGG(+ACTCAC)GATCCTTGGCACATTTTGAGAGTCTCCCAAC NGG 10 8 17 8 24 0 100 5 10
    240605003958_7gf4 ACACTCAAAGGAAAAAAAGTAGTTGAAGCTTG(+ACTCAC)GTACCTTTAAAGTTTGTGTACCTTACAGACA NGG 10 8 17 8 24 0 100 5 10