Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605004031_bjgz TATTCTCAGGGCTAAGCCCAGGAAAACTAAAG(+ACTCAC)GATCCAGTTCTAGAACTGGAGAGAAACCAAC NGG 10 8 17 8 24 0 100 5 10
    240605004029_gycf CTAAGTGGTTCACAACCACCTAGAATCCAGAG(+ACTCAC)GATCCGATATCCTCTTCTGGTATCCGTGGGC NGG 10 8 17 8 24 0 100 5 10
    240605004028_t08y GGGGTGCCCACGGATACCAGAAGAGGATATCG(+ACTCAC)GATCCTCTGGATTCTAGGTGGTTGTGAACCA NGG 10 8 17 8 24 0 100 5 10
    240605004026_nm28 AGGAAAAGTAAGGGGGGGACAAGTCTGAGGAG(+ACTCAC)GATCCAGTGGCCCGAAACCCCAGTCCCCACC NGG 10 8 17 8 24 0 100 5 10
    240605004024_cm7b CTGTGGGTGGGGACTGGGGTTTCGGGCCACTG(+ACTCAC)GATCCTCCTCAGACTTGTCCCCCCCTTACTT NGG 10 8 17 8 24 0 100 5 10
    240605004023_u6ud CCCTAGGTTGTTCCCAGGGCTGCAAAGCGTAG(+ACTCAC)GTACCAACACTTTCTTGTTCCCCTCCAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605004022_a8on CACCCTTCCTGGAGGGGAACAAGAAAGTGTTG(+ACTCAC)GTACCTACGCTTTGCAGCCCTGGGAACAACC NGG 10 8 17 8 24 0 100 5 10
    240605004020_43km GAGGGAGGTGATGCCTCGGAACAATAAGGATG(+ACTCAC)GATCCCTTCCTTCAACCCATGCTAGGGGAGA NGG 10 8 17 8 24 0 100 5 10
    240605004018_ljyv TTCCCTCTCCCCTAGCATGGGTTGAAGGAAGG(+ACTCAC)GATCCATCCTTATTGTTCCGAGGCATCACCT NGG 10 8 17 8 24 0 100 5 10
    240605004016_5g0s GGGGGCTAGAGCTCCCTTCTGGAATTCCAGAG(+ACTCAC)GATCCTGTATGGAAGTGGGGCTGGACCATGG NGG 10 8 17 8 24 0 100 5 10