Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605003957_qve8 CCAAATGTCTGTAAGGTACACAAACTTTAAAG(+ACTCAC)GTACCAAGCTTCAACTACTTTTTTTCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605003955_y90c ACCTGGAGTCAGGAGTAGGCTACAGGTCTCAG(+ACTCAC)GATCCCTGCCAAGCAAAGACAACAAATGCCT NGG 10 8 17 8 24 0 100 5 10
    240605003954_nqm9 GGGGCAGGCATTTGTTGTCTTTGCTTGGCAGG(+ACTCAC)GATCCTGAGACCTGTAGCCTACTCCTGACTC NGG 10 8 17 8 24 0 100 5 10
    240605003951_3q4j AGGGGGAAGAGCTGGGGACAAGCTCCTCCCTG(+ACTCAC)GATCCCTGTGGATTTTGAAAAGATACTATTT NGG 10 8 17 8 24 0 100 5 10
    240605003950_q6cy AATAAAAATAGTATCTTTTCAAAATCCACAGG(+ACTCAC)GATCCAGGGAGGAGCTTGTCCCCAGCTCTTC NGG 10 8 17 8 24 0 100 5 10
    240605003948_wlwf AAAATGCCACGGTCCTGCTCTGGTCAATAAAG(+ACTCAC)GATCCTTTGAAGATATGCAAGTGGTGGTCTT NGG 10 8 17 8 24 0 100 5 10
    240605003947_ek6z TAAGGAAGACCACCACTTGCATATCTTCAAAG(+ACTCAC)GATCCTTTATTGACCAGAGCAGGACCGTGGC NGG 10 8 17 8 24 0 100 5 10
    240605003945_b0dr TAATAGGGGTGGGGAATCAATAAACATGAATG(+ACTCAC)GTACCTACTACTCCTTTCTTTCCTCATTAAG NGG 10 8 17 8 24 0 100 5 10
    240605003944_8tm3 GTACTCTTAATGAGGAAAGAAAGGAGTAGTAG(+ACTCAC)GTACCATTCATGTTTATTGATTCCCCACCCC NGG 10 8 17 8 24 0 100 5 10
    240605003942_o2uq TAATCCCACTACTTGGGAGGCAGAGGCAGGGG(+ACTCAC)GATCCCTTGAGTTCAAGGCCAGTCTGGTCTA NGG 10 8 17 8 24 0 100 5 10