Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605004045_kaar ATCTCTCATGGTGTTCACCTTCATCTCTCATG(+ACTCAC)GTACCTCATACCTGAAATGTCTCATGGGTCA NGG 10 8 17 8 24 0 100 5 10
    240605004044_ghh2 TTTTTAAGAAGTATGATTCCCAGGCTCTCAAG(+ACTCAC)GATCCTACAGACTTGTTGGCATATAAAGGGC NGG 10 8 17 8 24 0 100 5 10
    240605004043_0wjg CCATAGCCCTTTATATGCCAACAAGTCTGTAG(+ACTCAC)GATCCTTGAGAGCCTGGGAATCATACTTCTT NGG 10 8 17 8 24 0 100 5 10
    240605004042_yzcf CACTGCCCTCAGAACTCAGGGTTTGGGGCAAG(+ACTCAC)GATCCAGGCACGATAGGGCCCAACGGCTCGC NGG 10 8 17 8 24 0 100 5 10
    240605004040_pytp CAGCCGCGAGCCGTTGGGCCCTATCGTGCCTG(+ACTCAC)GATCCTTGCCCCAAACCCTGAGTTCTGAGGG NGG 10 8 17 8 24 0 100 5 10
    240605004039_7jz4 TCCAGCAAACATTTATTGATAACCCAAGTAAG(+ACTCAC)GTACCCTGCAGATGTAGAAAAAAGCATCATG NGG 10 8 17 8 24 0 100 5 10
    240605004037_4637 CCATCCATGATGCTTTTTTCTACATCTGCAGG(+ACTCAC)GTACCTTACTTGGGTTATCAATAAATGTTTG NGG 10 8 17 8 24 0 100 5 10
    240605004036_897r CTGTAAATATAATTTATTGAGGCCTGTAGTTG(+ACTCAC)GTACCAGGGCCTTTATTCTGTTTGCATTTCC NGG 10 8 17 8 24 0 100 5 10
    240605004034_pla5 AAGTCGGAAATGCAAACAGAATAAAGGCCCTG(+ACTCAC)GTACCAACTACAGGCCTCAATAAATTATATT NGG 10 8 17 8 24 0 100 5 10
    240605004032_4pos TGCTTGTTGGTTTCTCTCCAGTTCTAGAACTG(+ACTCAC)GATCCTTTAGTTTTCCTGGGCTTAGCCCTGA NGG 10 8 17 8 24 0 100 5 10