Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605003940_i71c TTAGTTAGACCAGACTGGCCTTGAACTCAAGG(+ACTCAC)GATCCCCCTGCCTCTGCCTCCCAAGTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605003939_nm77 CTCCTGGCTATAGCCCTCATCTCCAATTTTAG(+ACTCAC)GATCCCTTAAGACTTGACTACACCTTTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605003937_h44u AGGTCAGGAAAAGGTGTAGTCAAGTCTTAAGG(+ACTCAC)GATCCTAAAATTGGAGATGAGGGCTATAGCC NGG 10 8 17 8 24 0 100 5 10
    240605003935_32n4 TTGGACTTCAATAGAAACGATGTGGTGTCCTG(+ACTCAC)GTACCATGGCCAGACCCAGCACACGGGGTTC NGG 10 8 17 8 24 0 100 5 10
    240605003933_mxk4 CGGGCGAACCCCGTGTGCTGGGTCTGGCCATG(+ACTCAC)GTACCAGGACACCACATCGTTTCTATTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605003931_ilsm CCCAAGCCGGTTCCTTACCGCGGCCTGAGCCG(+ACTCAC)GATCCTACCATCCCTTTGATGGAACAGCTGA NGG 10 8 17 8 24 0 100 5 10
    240605003930_esh4 CACATTCAGCTGTTCCATCAAAGGGATGGTAG(+ACTCAC)GATCCGGCTCAGGCCGCGGTAAGGAACCGGC NGG 10 8 17 8 24 0 100 5 10
    240605003928_j7zo TCAAGACATTGTAGTGAGGGACCCACAAATGG(+ACTCAC)GTACCTTTTTTTTTCCAGTAGGCCTTGCCAC NGG 10 8 17 8 24 0 100 5 10
    240605003927_jvom TGTTAGTGGCAAGGCCTACTGGAAAAAAAAAG(+ACTCAC)GTACCCATTTGTGGGTCCCTCACTACAATGT NGG 10 8 17 8 24 0 100 5 10
    240605003926_st73 CCCATGCAACCTAAGAGTCCATAAATTGTTTG(+ACTCAC)GATCCATCAATCGTGATATTTAGTAAGCTCA NGG 10 8 17 8 24 0 100 5 10