Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605003924_4nw4 ATTTATGAGCTTACTAAATATCACGATTGATG(+ACTCAC)GATCCAAACAATTTATGGACTCTTAGGTTGC NGG 10 8 17 8 24 0 100 5 10
    240605003923_spni ACATTGTCAACCACAGACCACTGCTAAAATTG(+ACTCAC)GATCCTTTGCTTACACCACAGACTCTGAACA NGG 10 8 17 8 24 0 100 5 10
    240605003922_nuor GCGTTTGTTCAGAGTCTGTGGTGTAAGCAAAG(+ACTCAC)GATCCAATTTTAGCAGTGGTCTGTGGTTGAC NGG 10 8 17 8 24 0 100 5 10
    240605003920_20qu AGTGGTGAAGAGCACTTGTTAGTTTTCCAAAG(+ACTCAC)GATCCAGGTTTGATATCCAAGACCTACAGAG NGG 10 8 17 8 24 0 100 5 10
    240605003918_cubf AGGCTCTCTGTAGGTCTTGGATATCAAACCTG(+ACTCAC)GATCCTTTGGAAAACTAACAAGTGCTCTTCA NGG 10 8 17 8 24 0 100 5 10
    240605003915_0chn GAGCCCGCGCGCCCCGCTGCCCTCCTCGCCCG(+ACTCAC)GATCCCTCTCCTGCCCTCTGACTGGAGTCTC NGG 10 8 17 8 24 0 100 5 10
    240605003914_ss8u GTGTAGAGACTCCAGTCAGAGGGCAGGAGAGG(+ACTCAC)GATCCGGGCGAGGAGGGCAGCGGGGCGCGCG NGG 10 8 17 8 24 0 100 5 10
    240605003911_jz3s CACCCTGGAGCAGCTAGGGGTTCACGGACCTG(+ACTCAC)GATCCTACACAGATCCCAGGTTCCCCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240605003910_jfeu GATGGGAGGAGGGGAACCTGGGATCTGTGTAG(+ACTCAC)GATCCAGGTCCGTGAACCCCTAGCTGCTCCA NGG 10 8 17 8 24 0 100 5 10
    240605003908_bzx6 CTCTGGGCTCAACAGCCCTGGAGAAGGACGAG(+ACTCAC)GATCCCCATCCTCCAGCAAGTCCCAACACAT NGG 10 8 17 8 24 0 100 5 10